青花菜种质的ISSR分子标记研究

Study on Sprouting Broccoli Resources by ISSR Markers

利用ISSR标记对14份青花菜材料和1份油菜参照材料进行了基因组多态性分析,从60条引物中筛选出12条可扩增出清晰且具多态性条带的引物.共扩增出108条带,其中84条具有多态性,多态性百分率为77.78%,被测材料的ISSR标记多态性较高.其中每条引物可扩增出4-17条多态性带,平均7条.ISSR标记遗传相似性系数（GS）变异范围为0.531 2-0.859 4,平均值为0.744 4±0.073 4.通过UPGMA进行聚类分析,青花菜材料和油菜参照材料彼此间区分明显,14份青花菜材料聚为2类.结果表明,ISSR标记可以有效地评价青花菜遗传分化并为青花菜种类的鉴别提供一定的理论依据.

14 sprouting broccoli and one rape reference were used as materials for analyzing their genome polymorphism by ISSR markers. 12 primers selected from 60 primers were used for ISSR amplification. A total of 108 bands were generated,of which 84 bands were polymorphic bands （the percentage of polymorphic band,PPB= 77.78 %）. Each primer was able to generate 4 to 17 polymorphic bands and average was 7. Genetic similarity values between samples varied from 0. 531 2 to 0. 859 4, mean 0. 744 4 ± 0.073 4. The clustering dendrogram was constructed by UPGMA method. Sprouting broccoli materials and rape reference were distinguished from each other. 14 sprouting broccoli materials were divided into two major groups. As this study indicated above,we suggested that ISSR method was able to evaluate genetic variation of sprouting broccoli effectively and was helpful to the classification of sprouting broccoli.