镁剂对大鼠蛛网膜下腔出血后血管内皮生长因子表达的影响

The effect of MgSO_4 on expression of VEGF after subarachnoid hemorrhage of SD rats

目的观察硫酸镁对大鼠蛛网膜下腔出血(SAH)模型脑内内皮细胞生长因子(VEGF)的影响。方法将96只SD大鼠随机分为SAH组、假手术组、SAH加镁组、假手术加镁组建立实验动物模型。分别于相应时间点采用免疫组化技术分析大鼠脑内VEGF分布情况。结果假手术组和假手术加M镁组均可见到少量VEGF的表达,两者差异无显著性。在蛛网膜下腔出血组24h明显表达,3d表达强度进一步增高,7d达高峰,14d下降至正常。其阳性细胞主要分布在大脑皮层,基底节区、海马等部位也有较多。其细胞类型主要为神经细胞、胶质细胞、血管内皮细胞等。在SAH后的不同时间点免疫组织化学结果为:SAH加镁组大鼠VEGF的表达明显高于SAH组。结论硫酸镁促进了VEGF的表达,其通过促进VEGF的表达参与了继发性脑损伤的保护机制。

Objective To explore effect of MgSO4 on expression of vascular endothelial growth factor （VEGF） after subaraehnoid hemorrhage （SAH） of SD rats. Methods Ninety - six SD rats were divided randomly into four groups including： pseudo - operated group; SAH model group; pseudo - operated groups dealed with MgS04, SAH model group dealed with MgSO4. VEGF was measured by radio immunoassay respectively at different time, Results Compared with those in SAH model group, the level of expression of VEGF was increased in SAH model group dealed with MgSO4. Conclusion Magnesium can increase the level of VEGF expression.