黄瓜自根苗、嫁接苗根系活力、净光合速率和蛋白质表达差异研究

Study on root activity,net photosynthetic rateand proteome of none-grafted and grafted cucumber(Cucumis satiuvs L.)

将黄瓜津优1号、津优1号与黑子南瓜的嫁接苗和黑子南瓜种在网室中的消毒泥炭钵内,用LI-6400光合系统和2D-E电泳技术分析了这3种处理的根系活力、净光合速率和蛋白质的表达差异。结果表明,黑子南瓜的根系活力强于嫁接苗黄瓜,嫁接苗黄瓜的根系活力又强于自根苗黄瓜。在光量子通量密度低于500μmol.m-2.s-1时,嫁接黄瓜和黑子南瓜的净光合速率高于自根苗黄瓜,当光通量子密度超过500μmol.m-2.s-1后,嫁接黄瓜和黑子南瓜的净光合速率则低于自根苗黄瓜。当CO2浓度低于400μmol.mol-1时,嫁接黄瓜与自根苗的光合速率相当,CO2浓度超过400μmol.mol-1后,嫁接黄瓜的光合速率超过了自根苗。黑子南瓜的光合速率在50~600μmol.mol-1的CO2浓度范围内最高,然后下降,并间于嫁接黄瓜和自根苗黄瓜之间。嫁接的津优1号黄瓜有5个蛋白质的表达量增强了,同时也产生了5个新的蛋白质。

The cucumber（Cucumis Satiuvs L.）Jinyou 1,grafted Jinyou 1 with figleaf gould stock and figleaf gould（C.ficifolia） were cultivated in turf pot in net house,and LI-6400 photosynthetic system and 2D-E technology were used to study the root activity,net photosynthetic rate and proteome.The results showed that the root activity of figleaf gould was the strongest among 3 treatments,and grafted cucumber was stronger than none-grafted one.The grafted and figleaf gould had higher net photosynthetic rate than the none-grafted when the photon flux density was under 500 μmol·m-2.s-1,but the phenomena was reversed when the photon flux density was over 500 μmol·m-2.s-1.When CO2 concentration was less than 400 μmol·mol-1,the grafted had similar net photosynthetic rate with the none-grafted,but the net photosynthetic rate of the grafted surpassed the none-grafted when the CO2 concentration was over 400 μmol·mol-1.The net photosynthetic rate of figleaf gould was the highest as the CO2 concentration was between 50 and 600 μmol·mol-1,but it declined when the CO2 concentration was over 600 μmol·mol-1,and lied between grafted and none-grafted cucumber.There were 5 protein spots of grafted cucumber that showed more in quantity,and another 5 new protein spots were also induced in grafted cucumber compared with the none-grafted one.