抗卵巢抗体的检测及其抗生育作用的研究

Detection of anti ovum antibodies and their effects against fertility

用ＴｒｉｔｏｎＸ－１００自人卵巢组织匀浆中提取得卵巢抗原（ＯＡｇ），建立了检测抗卵巢抗体（ＡｏＡｂ）的ＥＬＩＳＡ法。发现卵巢功能早衰、不孕症、流产患者ＡｏＡｂ水平与检出率均显著高于正常生育妇女。与ＡｏＡｂ阴性患者比较，ＡｏＡｂ阳性患者基础体温呈双相型者较少，呈黄体功能不足型者较多；Ｂ超监测发现排卵者减少，不排卵者增多；雌二醇（Ｅ２）、孕酮（Ｐ）水平显著降低。经ＡｏＡｂ阳性血清的γ球蛋白作用后，体外培养的卵巢组织细胞分泌ＩＬ－１β、ＴＮＦ－α、ｓＩＬ－２Ｒ显著增多，而分泌Ｅ２与Ｐ显著减少。研究结果提示，ＡｏＡｂ具有潜在的抗生育作用。

An ELISA for detection of anti ovum antibodies(AoAb) was developed based on extracting of the ovum antigens (OAg) from the homogenate of human ovum tissue by Triton X 100. It was found that the levels as well as the positive rate of AoAb in the women with premature decrepitude of ovum function, infertility and abortion were significantly higher than those in normal fertilizable women, Compared with the patients with negative AoAb, double phase of basal body temperature was presented in fewer but dysfunction of corpus in more patients with positive AoAb, and ovulation occurred by monitoring of B type ultrasonography in fewer but nonovulation in more patients. Both of the levels of estradiol (E2) and progesteron(P) in these patients significantly decreased. Following the action of γ globulin from AoAb positive sera, the ovum tissue cells cultivated in vitro secreted notably more IL 1β, TNA α and sIL 2R but less E2 and P. These results indicate AoAbs possess potential effect against fertility.