PCR 和探针杂交技术检测口腔粘膜疾病中人乳头瘤病毒的核酸

Examination of human popillomavirus of oral mucous membrane diseases by PCR and probe hybridization

联合应用ＰＣＲ和核酸杂交的方法对１５份口腔粘膜疾病患者样品进行了分析。用ＰＣＲ技术测ＨＰＶ－１６为３份阳性，ＨＰＶ－１８为７份阳性；然后用探针杂交检测ＰＣＲ产物其ＨＰＶ－１６，３份阳性，ＨＰＶ－１８，１０份阳性。以上结果表明ＨＰＶ与口腔粘膜疾病有一定的关系；用ＰＣＲ扩增法和探针杂交法联合检测可提高检测率。

The correlation of Human Popillomavirus (HPV) with oral mucous membrane diseases was investigated. Method: HPV16, HPV18 was detected in 15 cases by using polymerase chain reaction(PCR) and dot blot hybridization. Results: 3 cases showed positive in HPV16 by both methods. 7 cases showed positive in HPV18 by PCR and 10 cases showed positive by dot blot hybridization. Conclusion: The results indicated correlation to HPV with oral mucous membrane disease. A high sensitivety and specifity was detected by using PCR and hybridization methods.