华蟾毒精对小鼠免疫功能影响的体外研究

Study on effects of cinobufagin on the immunologic function of mice in vitro

AIM: To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufonis, a famous traditional Chinese medicine in vitro. METHODS: Proliferation of the splenic lymphocytes in Balb/c mice was measured by MTT assay. The metaboilic level and phagotrophic activity of PMΦ were also determined by MTT assay. The expressions of CD4 and CD8 and cell cycle of the splenic lymphocytes were detected by flow cytometry. RESULTS: Administration of cinobufagin or coadministration with Con A or LPS increased the cell proliferation and the percentage of S phase cells in the splenic lymphocytes at a dose-dependent manner(P<0.05 or P<0.01). Cinobufagin also enhanced the metabolic capability of MTT and the phagocytosis function of PMΦ(P<0.05 or P<0.01). In addition, it was found that the single administration of cinobufagin or coadministration with Con A or LPS also could increase expressions of CD4 and CD8 in T lymphocytes(P<0.05 or P<0.01). CONCLUSION: Single administration of cinobufagin or coadministration with Con A or LPS could enhance the reactive ability to the splenic lymphocytes and PMΦ in mice. The data indicate that the immunoregulatory effect of cinobufagin is related to the increased expression of CD4+ and CD8+ in DP (double positive) phenotype and S phase cells of T lymphocytes.

AIM： To investigate the immunoregulatory effects of cinobufagin from the chloroform extract of Venenum Bufonis, a famous traditional Chinese medicine in vitro. METHODS： Proliferation of the splenic lymphoeytes in Balb/c mice was measured by MTT assay. The metaboilie level and phagotrophie activity of PMφ were also determined by M3T assay. The expressions of CD4 and CDs and cell cycle of the splenic lymphoeytes were detected by flow cytometry. RESULTS： Administration of einobufagin or eoadministration with Con A or LPS increased the cell proliferation and the percentage of S phase cells in the splenic lymphocytes at a dose-dependent manner （P 〈 0.05 or P 〈 0.01 ）. Cinobufagin also enhanced the metabolic capability of M3T and the phagoeytosis function of PMφ （ P 〈 0.05 or P 〈 0.01 ）. In addition, it was found that the single administration of einobufagin or coadministration with Con A or LPS also could increase expressions of CD4 and CDs in T lymphocytes（ P 〈 0.05 or P 〈 0.01 ）.