快速灵敏的LC-MS/MS方法检测人血浆中普伐他汀的浓度

Rapid and sensitive LC-MS/MS method for determination of Pravastatin in human plasma

AIM: To establish a LC-MS/MS method for determination of Pravastatin in human plasma. METHODS: After addition of rosuvastatin (internal standard) with liquid-liquid extraction, the analysis involved an Intersil ODS-3(2.1 mm×50 mm,5 μm)column. The mobile phase consisted of acetonitrile:1 mmol ammonium formate (include 0.1% formic acid)(60∶40,v/v),the flow rate was 0.2 mL/min, the column temperature was set at 40 ℃. ESI+was performed in the MRM mode using target ions at m/z 448-328(Pravastatin)and m/z 483-271(rosuvastatin). RESULTS: The peak area of Pravastatin in plasma showed good linearity within the range between 0.957-510.000 ng/mL,r2=0.999. The lower limit of quantitation (LLOQ) of pravastatin in plasma was 0.957 ng/mL. The extracted recovery was >75%, The relative recovery was 99%-115%,the intra-and inter-day RSD were <15%. CONCLUSION: This method is sensitive,simple and accurate, it can be used for determination of Pravastatin sodium tablets serum concentrations.

AIM： To establish a LC-MS/MS method for determination of Pravastatin in human plasma. METHODS： After addition of rosuvastatin （ internal standard） with liquid-liquid extraction, the analysis involved an Intersil ODS-3 （2.1 mm × 50 mm, 5 μm）column. The mobile phase consisted of acetonitrile： 1 mmol ammonium formate （include 0.1% formic acid） （60 ： 40, v/v）, the flow rate was 0.2 mL/min, the column temperature was set at 40 ℃. ESI ＋ was performed in the MRM mode using target ions at m/z 448- 328（ Pravastatin） and m/z 483- 271 （ rosuvastatin）. RESULTS： The peak area of Pravastatin in plasma showed good linearity within the range between 0. 957 - 510. 000 ng/mL, r^2 = 0. 999. The lower limit of quantitation （LLOQ） of pravastatin in plasma was 0. 957 ng/mL. The extracted recovery was 〉 75 %, The relative recovery was 99% - 115%, the intra-and inter-day RSD were 〈 15%.