国产微柱凝胶抗人球蛋白不规则抗体筛检卡在红细胞不规则抗体筛检中的应用

Application of Domestic Anti-globulin Irregular Antibodies Microcolumn Gel Test Screening Cards in Erythrocytic Irregular Antibodies Screening

目的探讨国产微柱凝胶抗人球蛋白不规则抗体筛检卡(以下简称:筛检卡)对血清(浆)中不规则抗体的检出效能。方法用筛检卡与凝聚胺法同时筛检IgG类抗C、抗c、抗D、抗E4种标准抗血清,并比较结果;取334份有免疫史或怀疑有新生儿溶血病的患者血清(浆),同时用两法筛检,结果用统计学处理。结果两法对IgG类4种标准抗血清的筛检结果(阴性、阳性)完全一致;但两法阳性凝集强度有部分不同。334份血清(浆)筛检卡检出9例不规则抗体阳性,凝聚胺法检出7例,两法不规则抗体检出差异无统计学意义(P>0.05),在两法共同检出的7例中,有2例筛检卡凝集强度高于凝聚胺法。结论国产筛检卡血清(浆)不规则抗体筛检结果可靠、操作简便,可常规应用,尤其适用于大批量标本的筛检。

Objective To explore the detection efficacy of domestic anti-globulin irregular antibodies mieroeolumn gel test screening cards（ abbreviated to screening cards ） for screening erythroeytie irregular antibodies in serum （plasma）. Methods Four kinds of IgG antibodies standard antiserums including anti-C, anti-e, anti-D and anti-E were screened using screening cards and polybrene kits simultaneously. 334 serum（plasma） samples from patients with immune disease history or suspected hemolytic disease of the newborn were screened using screening cards and polybrene kits simultaneously. All results were analyzed by statistics method. Results （ 1 ） The screening results （ positive or negative） of two methods for first contrasting experiment were totally uniform except for the different intensity of positive agglutination partly. （2）9 irregular antibodies positive serum （plasma） samples were screened out using screening cards and 7 were screened out using polybrene kits for next contrasting experiment. The distinction between two methods for screening irregular antibodies was no significant difference（ P 〉 0.05 ） and the 2 samples among 7 samples screened out by two methods had higher agglutination intensity detected by screening cards than that by polybrene method. Conclusions Domestic anti-globulin irregular antibodies mieroeolumn gel test screening cards has advantages such as reliable results and convenient manipulation, it can be used for conventional practice and especially for screening a large number of samples.