“益肾清火”方对牙周炎大鼠牙槽骨重建的影响

The impact of Yishenqinghuo recipe on alveolar bone reconstruction of rats with experimental periodontitis

目的:观察中药"益肾清火"方对牙周炎大鼠牙槽骨重建的影响。方法:选择12个月龄SD大鼠,分为A、B、C、D共4组,每组各6只动物。A组为空白对照组,B组为牙周炎造模组,C组为造模后灌服中药高剂量组,D组为造模后灌服中药等效剂量组。牙周炎造模成功后,A、B组动物灌喂生理盐水,C、D组动物灌喂中药3个月。方块截取各组动物牙槽骨,制作硬组织切片,行苦味酸-品红染色后,分析各组牙槽骨骨量、骨结构及骨转换参数的差异。采用SAS6.04软件包对数据进行单因素方差分析。结果:牙周炎大鼠灌服"益肾清火"方3个月后,与B组相比,C、D组类骨质形成明显增多,骨小梁密度较高、游离末端数少;A、B、C、D各组骨小梁面积百分数和结点末端比分别为:75.24±3.82/1.49±0.12、45.78±6.70/0.48±0.08、73.33±4.20/1.33±0.06和67.69±2.83/1.26±0.10。B组的2项参数均低于其余3组,统计学上有显著性差异(P<0.01)。各组类骨质面积(变量经转换)分别为(88.44±7.52)μm2、(145.37±13.91)μm2、(211.10±22.96)μm2和(201.22±24.75)μm2。A组类骨质面积比其余3组低,而B组又低于C、D2组,统计学上有显著性差异(P<0.01)。结论:中药"益肾清火"方有助于增加实验性牙周炎大鼠牙槽骨骨量,改善其骨质结构,促进骨的修复重建。

PURPOSE： To evaluate the impact of Yishenqinghuo recipe on alveolar bone reconstruction of rats with experimental periodontitis. METHODS： 12-months old Spague-Dawley rats were selected for the study. All rats were randomly divided into 4 groups. Group A： control group （with no periodontitis model, fed with the same dosage of saline as Group D）; Group B： model group （with periodontitis model, fed with the same dosage of saline as Group D）; Group C： high-dosage group （with periodontitis model, fed with doubling dosage of medicine as Group D）; Group D： equivalentdosage group （with periodontitis model, fed with clinical equivalent effective dosage of medicine）. After being gavaged with medicine/saline for 3 months, the alveolar bone were collected to make undecalcified histological slices and evaluate the changes of alveolar bone histomorphometric parameters. All the results were analyzed by ANOVA, with the use of SAS 6.04 software package. RESULTS： Compared with group B, the formation of osteoid were increased and had less free ending trabecular in group C and D. The percent age of trabecular area （%Tb.Ar） and node-terminus ratio （NTR） of alveolar bone for this 4 groups were 75.24±3.82/1.49±0.12, 45.78±6.70/0.48±0.08, 73.33±4.20/1.33±0.06 and 67.69±2.83/ 1.26±0.10,respectively. This two parameters of group B were much lower than that in other three groups （P〈0.01）. At the same time, the NTR in group D was significantly lower than that in group A （P〈0.01）. The osteoid area （Os.Ar） of different groups were （88.44±7.52）μm^2, （145.37± 13.91）μm^2, （211.10±22.96）μm^2 and （201.22±24.75）μm^2（transformation variables）, respectively. The Os.Ar of group A was lowest, and that in group B was lower than group C and D, there were significant differences（P〈0.01）. CONCLUSIONS： From this study, we conclude that Yishenqinghuo recipe is in favor of enhancing alveolar bone quantity, improving bone structure and reconstructing bone loss of rats with experimental periodontitis. Supported by National Key Technologies R＆D Program （Grant No.2004BA726） and Natural Science Foundation of Shanghai Municipality （Grant No. 03ZR14081）.