大蒜多糖C对免疫性肝损伤小鼠外周血淋巴细胞亚群的影响

Effects of Garlic Polysaccharide C on lymphocyte subsets in the peripheral blood of mice with immunologic liver injury

目的:研究大蒜多糖C对免疫性肝损伤小鼠外周血淋巴细胞亚群的影响。方法:采用尾静脉注射卡介苗加脂多糖(BCG+LPS)建立免疫性肝损伤模型,应用流式细胞术测量小鼠外周血淋巴细胞亚群的百分比和CD4+/CD8+比值。结果:模型组T淋巴细胞、CD8+T细胞百分比值较正常对照组明显升高(P<0.01),B淋巴细胞百分比值、CD4+/CD8+比值明显下降(P<0.01),CD4+T细胞百分比也有减少(P<0.05),NK1.1+T细胞百分比无显著变化。大蒜多糖C(800mg/kg ig,qd×12)治疗能使BCG+LPS免疫性肝损伤小鼠外周血T、B、CD4+T和CD8+T淋巴细胞百分比显著下降,而CD4+/CD8+比值显著上升,恢复到正常水平,对NK1.1+T细胞百分含量则无明显影响。结论:大蒜多糖C能全面抑制免疫性肝损伤小鼠的免疫功能,并促使机体建立新的免疫平衡,从而对免疫性肝损伤产生保护作用。

Objective： To study the effects of Garlic Polysaccharide C on the peripheral-blood lymphocyte subsets in mice with immunologic liver injury. Method ： The immunological liver damage animal model was reproduced by injection via the tail vein of BCG （ Bacilli Calmette Guein） and lipopolysaccharide（LPS） into mice. The peripheral-blood lymphocyte subsets＇ percentages and the CD4^＋/CD8^＋ ratio was measured by the flow cytometry. Results： Compared with normal control group, the percentages of the T lymphocyte and the CD8^＋ T lymphocyte in the peripheral-blood in immunological liver damage mice were siginificantly increased（ P 〈 0.01 ）. however, the percentages of the B lymphocyte, CD4^＋ T lymphocyte and the ratio of CD4^＋/CD8^＋ decreased（ P 〈 0.05 or P 〈 0.01 ）. Garlic polysaccharide C at dose of 800 mg/kg （ ig,qd × 12） decreased the percentages of T,B,CD4^＋ T and CD8^＋ T lymphocyte in the peripheral-blood in mice treated with BCG ＋ LPS. however, the CD4^ ＋/CD8^＋ ratio increased to the normal level. The percentages of the NK1.1 ^＊ T lymphocyte showed no obvious difference between control, BCG ＋ LPS liver injury model and Garlic Polysaccharide C groups. Conclusions ： Garlic Polysaccharide C can inhibit the immunologic activity, and establish a new immunologic balance, thereby produce protective effects on immunologic liver injury.