石榴皮提取物对大鼠实验性结肠炎的保护作用

Effects of pomegranate peel extract on ulcerative colitis induced by TNBS in rats

目的:研究石榴皮对大鼠实验性结肠炎组织损伤的保护作用及其机制。方法:用三硝基苯磺酸建立大鼠实验性结肠炎模型,灌胃停药2周后评价大鼠结肠粘膜损伤指数(CMDI),检测结肠组织髓过氧化物酶(MPO)、超氧化物歧化酶(SOD)谷胱甘肽过氧化物酶(GSH-PX)活性和丙二醛(MDA)含量。结果:石榴皮200、400、800mg/kg灌胃能不同程度降低结肠炎模型大鼠CMD I、MPO活性,减少MDA含量,提高SOD、GSH-PX活性。结论:石榴皮对TNBS所致大鼠实验性结肠炎有明显保护作用。

Objective：To study the protect effect of pomegranate peel extract on the colonic tissue with ulcerative colonitis induced by trinitrobenzene sulfonic acid （TNBS）in rats. Methods： Rat model of ulcerative colonitis was established by clyster of TNBS. After treatment with pomegranate peel for two weeks ,the colon mucosal damage index（CMDI） was evaluated, the activity of myeloperoxidase （MPO）, superoxide dismutase （SOD） , glutathione peroxidase（ GSH -PX） and the content of malondialdehyde（MDA） in the colon tissues were examined. Results： Pomegranate peel extract（200,400,800mg/kg）can decrease the values of CMDI ,the activity of MPO and the content of MDA, increase the activity of SOD and GSH - Px in a dose - depended manner. Conclusion： Pomegranate peel extract can relieve the inflammatory reaction and colonic injury in colonitis rats by anti -oxidation and wound repair.