Aβ1-40脑内注射对大鼠海马AchE和NOS1表达的影响及加减地黄饮子的干预作用

Interference effects of Extracts from Modified Decoction of Rehmanniae(加减地黄饮子) on the expressing of AchE and NOS1 in hippocampus of AD rats

目的:观察加减地黄饮子对β-淀粉样蛋白1-40(Aβ1-40)诱导的AD模型大鼠大脑海马组织AchE和NOS1表达的影响。方法:采用大鼠海马立体定向注射凝聚态Aβ1-40诱导老年性痴呆(Alzheimer's disease,AD)动物模型。采用免疫组织化学染色法和计算机图像分析技术测定海马区AchE和NOS1表达。结果:模型组(6.42±0.38)AchE表达与假手术组(6.74±0.47)比较无显著性差异。与模型组比较,加减地黄饮子组(3.77±0.29)AchE表达明显减少,作用效应较盐酸多奈哌齐(2.25±0.20)弱。与假手术组(5.74±0.39)相比,模型组大鼠海马区NOS1表达下降(2.38±030),与模型组比较,地黄饮子组NOS1的表达增加(4.43±0.40),作用优于盐酸多奈哌齐(3.56±0.27)。结论:加减地黄饮子可通过抑制AD模型大鼠海马组织AchE和上调AD模型大鼠海马组织NOS1表达而发挥抗AD的作用。

Objective： To observe the effects of Modified Decoction of Rehmanniae on expression of Ache 和 NOS1 protein in hippocampal in Alzheimer＇s disease rats induced by amyloid - β1 - 40 injection into brain. Methods： AD model in rats was induced by amyloid - β1 - 40 sterotaxis injection the bilateral hippocampus. Modified Decoction of Rehmanniae was geven by oral administration for 28 days. the expression levels of AchE 和 NOS1 protein in the hippocampus were measured by immunohistochemical SABC techniques. Results： the levels of AchE expression from t the Modified Decoction of Rehmanniae group （ 3.77 ± 0.29） was significantly lower than that in the model group （6.42 ± 0.38, p 〈 0.05 ） , and the levels of NOS1 expression from the model group （2.38 ± 030） was significantly Lower than that in the sham group （ 5.74 ± 0.39 ） , and that from the Modified Decoction of Rehmanniae group （4.43 ± 0.40 ） was significantly highter than in the model group （ p 〈 0.05 ）. Conclusions： Modified Decoction of Rehmanniae has sighificant inhibitory effects on expression of AchE and Up - regulateing NOS1 in hippocampus of AD rats, and could be used as an effective drug for AD