摘要
RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphic band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphic band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify T. matsutake and T. bakamatsutake at molecular level.
RAPD analysis was adopted to identify the Tricholoma matsutake and T. bakamatsutake. With the method of BSA, a polymorphie band of 870 bp linked to T. matsutake was amplified, and the SCAR marker was developed from the polymorphie band by cloning, sequencing and designing primers. The SCAR marker only presented in the individuals of T. matsutake, thus, it can quickly and accurately identify T. matsutake and T. bakamatsutake at molecular level.
出处
《林业科学》
EI
CAS
CSCD
北大核心
2007年第10期150-153,共4页
Scientia Silvae Sinicae
基金
国家自然科学基金资助项目(30260071)
关键词
松茸
假松茸
RAPD
SCAR
Tricholoma matsutake
Tricholoma bakamatsutake
RAPD
SCAR
