2004-2006年东莞市流行性感冒病毒监测结果及分析

Surveillance results and analysis of influenza virus in Dongguan City from 2004 to 2006

目的了解东莞市流行性感冒(流感)病毒感染流行情况。方法采集门诊疑似流感病人咽拭子分泌物,接种MDCK细胞,于CO2温箱中33.5℃分离培养。培养液血凝试验阳性标本进行分型鉴定。结果2004年412例中有42例分离出流感病毒,分离阳性率为10.19%;其中H3N2型24例,B型18例。2005年521例中有19例分离出流感病毒,分离阳性率为3.65%;其中H3N2型10例,H1N1为5例,B型4例。2006年945例中有105例分离出流感病毒,分离阳性率为11.11%;其中H1N1型37例,B型68例。病毒分离阳性率3年之间差异有统计学意义(χ2=24.42,P<0.01),2004与2006年分离阳性率高于2005年。结论2006年分离阳性率最高,2005年分离阳性率最低。2004年仅有H3N2与B型,2005年比2004年多了H1N1型,但2006年没有分离到H3N2,仅有H1N1和B型。2004与2005年以H3N2型流感病毒流行为主;2006年则以B型为主,其次为H1N1。

Objective The study was conducted to determine the prevalence of influenza （flu） infection in Dongguan City. Methods Swabs of clinic suspected influenza patients were collected for the vaccination of MDCK cells with isolation culture in CO2 tank with the temperature of 33.5℃. Hemagglutination test-positive samples were collected for identification. Results In 2004, there were 42 influenza virus isolated cases from 412 patients, with the positive isolation rate of 10.19%, of which there were 24 cases of H3N2 and 18 cases of B-type. In 2005, there were 19 influenza virus isolated cases from 521 patients, with the positive isolation rate of 3.65%, of which there were 10 cases of H3N2, 5 cases of H1N1 and 5 cases of B-type. In 2006, there were 105 influenza virus isolated cases from 945 patients, with the positive isolation rate of 11.11%, of which there were 37 cases of H1N1 and 68 cases of B-type. Positive isolation rates between the three years had significant differences （χ^2=24.42, P〈0.01）, and those in 2004 and 2006 were higher than those from 2005. Conclusions The positive isolation rate in 2006 was the highest, whereas that in 2005 was the lowest. In 2004, there were only B type and H3N2 isolated, while in 2005 H1N1 added in, there was no H3N2 isolated but H1N1 and B type in 2006. The prevailing B type, followed by H1N1, was found in 2006.