p27kip1基因转染联合化疗对胃癌细胞生长的影响

Effect of p27kip1 Gene Transfer Combined with Chemotherapy on Growth of Gastric Carcinoma Cells

背景：p27kipl是一种抑癌基因，早期研究显示p27kipl基因转染能明显抑制胃癌、食管癌细胞生长．表明该基因疗法可能是治疗胃癌、食管癌的新途径，深入阐明p27kipl的抑癌机制，可为p27kipl基因治疗胃癌、食管癌提供可靠的理论基础。目的：研究p27kipl基因转染联合化疗对胃癌细胞生长的影响。方法：将p27kipl重组腺病毒（Ad-p27kipl）转染胃癌细胞SGC-7901，免疫细胞化学法检测p27kipl的表达。加入5-氟尿嘧啶（5-FU）和顺铂（DDP）后．甲基噻唑基四唑（MrIT）法测定细胞生长抑制率,^3H-胸腺嘧啶核苷（^3H-TdR）掺入试验测定DNA的合成。结果：Ad-p27kipl转染胃癌细胞后，p27kipl表达明显增强。加入5-FU和DDP后，细胞生长抑制率显著增高．^3H-TdR掺人量显著降低（P〈0．01）。结论：p27kipl基因转染联合化疗能明显抑制胃癌细胞的生长。

Background：p27kipl is an anti-oncogene. Early studies have demonstrated that p27kipl gene transfer has significant inhibitory effect on growth of esophageal and gastric carcinoma cells. These results indicate that this gene transfer may be a novel strategy for the treatment of esophageal and gastric carcinoma, and clarification of its mechanism may provide theoretical basis for further p27kipl gene therapy. Aims： To investigate the effect of p27kipl gene transfer combined with chemotherapy on growth of gastric carcinoma cells. Methods： Recombinant adenovirus carrying p27kipl gene （Ad-p27kipl） was transfected into gastric carcinoma cells SGC-7901. Expression of p27kipl was detected by immunocytochemical technique. After treated with 5-fluorouracil （5-FU） and cisplatin （DDP）, respectively, growth inhibitory rate of gastric carcinoma cells was evaluated by methyl thiazolyl tetrazolium （MTT） assay, DNA synthesis of cells was determined by 3H-thymidine （3H-TdR） incorporation. Results： After transfected with Ad-p27kipl, expression of p27kipl of gastric carcinoma cells increased significantly. Growth inhibitory rate of gastric carcinoma cells treated with 5-FU and DDP increased obviously and 3H-TdR incorporation decreased （P〈0.01）. Conclusions： p27kipl gene transfer combined with chemotherapy can obviously inhibit the growth of gastric carcinoma cells.