摘要
本实验研究了水生环节动物星虫胶原蛋白的提取及纯化方法.SDS-PAGE电泳分析结果显示,所提取的胶原蛋白纯度较高.通过粘度测定分析星虫胶原蛋白的变性温度约35℃.酶解性质的研究表明,纯化的星虫胶原蛋白可被胰蛋白酶、胃蛋白酶和蛋白酶K水解.
In this study, the extraction and purification of collagen from Sipunculidae were studied. SDS-PAGE revealed that the exacted collagen was of high purity. The denaturation temperature of this collagen was nearly 35℃. In addition, the purified collagens could be degraded by trypsin, pepsin and proteinase K and the hydrolyzed products were proved of different molecular weights.
出处
《台湾海峡》
CAS
CSCD
北大核心
2007年第4期522-527,共6页
Journal of Oceanography In Taiwan Strait
基金
福建省自然科学基金资助项目(C0410036)
关键词
胶原蛋白
星虫
纯化
性质
collagen
Sipunculidae
purification
characteristics
