三草降压汤对自发性高血压大鼠降压作用的量效与时效关系研究

Study on Antihypertensive time-effect and dose-effect of Sancao Jiangya decoction on SHRs

目的:探讨三草降压汤(SCD)降压作用的量效与时效关系特点。方法:采用清醒状态下大鼠尾动脉间接测压法,分别测定三草降压汤小、中、大三个剂量组对自发性高血压大鼠不同时间点的血压。结果:SCD给药后2h血压开始降低,小、中、大3个剂量组的降压作用效果与剂量之间呈现出较好的相关性;给药后4h,大、中剂量组具有量效相关性,而小剂量组无降压作用。选择中剂量组进一步研究表明,在给药后1h即出现血压下降,并在1-4h内保持较稳定的降压效果,6h时血压开始回升,8h后基本恢复到用药前水平。结论:SCD选择中剂量10.4g生药量/kg体质量,给药后2h作为检测点较为合适。该结果为今后更为深入的研究SCD降压药效评价和作用机制奠定了基础。

Objective： To investigate the antihypertensive time-effect and dose-effect features of Sancao jiangya decoction （SCD）. Methods： The blood pressure of spontaneously hypertensive rats at different time points were measured after treatment with Sancao jiangya decoction of low, middle, high coficentration by tail-artery blood pressure measurement for conscious rats. Results： The blood pressure was decreased at 2 hours after drug taken, there were significant dose-effect relationship between the antihypertensive effect and the low, middle, high dose. At 4h after drug taken, the high, middle dose had dose-effect correlation, but the low-dose had no antihypertensive effect. Further research on the middle-dose shows that the blood pressure reduced at lh after drug taken, and the stable antihypertensive effect was keeping during 1-4h, the blood pressure began to rise at 6h, and got back to the level before drug taken at 8h. Conclusion： To choose the Middle-dose （10.4g crude drug/kg body weight） and 2h after drug taken is appropriate for SCD＇s use. This result laid a substantial foundation for further research on effects evaluation and mechanism of antihypertensive medicine.