三种分离大鼠主动脉内皮细胞方法的比较研究

Compared Study for Three Methods to Isolate Endothelial Cells from Rat Aorta

血管内皮细胞由于具有多种生物学功能而成为体外研究血管病理生理的良好载体。虽然人脐静脉内皮细胞易于获得和培养，已被广泛用于血管内皮的研究，但其来源于静脉，对于很多发生在动脉的疾病，如动脉粥样硬化等的研究并不可行，所以分离和培养动脉内皮细胞非常重要。

Objective: To develop a effective method with good reproducibility to isolate primary rat aortic endothelial cells(RAECs).Method: Enzyme digestion, planting loop method with moment heat treatment and planting clump method were used to isolate the RAECs. Cultured,purified and subcultured RAECs,then observed the cell growth and morphology and identified the RAECs by immunohistochemical method. Results: Using planting clump method, RAECs could emigrate from explants for 5~7 d and reached monolayer cobblestone-like for 14 d. RAECs were identified by immunohistochemical method, the positive reaction is showed in the cytoplasm, the rate of positive cells were 52.3 ±10.6%.Conclusion: Compared with enzyme digestion and planting loop method with moment heat treatment, planting clump method has more convenience and better reproducibility.