丹参对左肾静脉狭窄大鼠肾脏氧化应激及组织纤维化的影响

Effect of Salvia Miltiorrhiza on Renal Oxidative Stress and Tissue Fibrosis of Left Renal Vein Constriction Rats

目的:探讨丹参治疗左肾静脉狭窄大鼠肾脏过氧化损伤和纤维化的效果,为临床药物治疗左肾静脉受压综合征提供实验依据。方法:将大鼠分为3组,假手术组、模型组和丹参治疗组。采用左肾静脉不全结扎的方法建立大鼠左肾静脉狭窄模型,用丹参进行干预,于治疗6周后处死动物,取肾组织制备匀浆查超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量,免疫组化法检测转化生长因子-β1(TGF-β1)和I型纤溶酶原激活物抑制因子(PAI-1)的表达。结果:模型组大鼠较假手术组左肾组织MDA含量显著增高,SOD活性显著降低,TGF-β1和PAI-1表达增加。丹参治疗可降低MDA含量,提高SOD的活性,减少TGF-β1和PAI-1的表达。结论:左肾静脉狭窄大鼠肾脏氧自由基生成增多,抗氧化能力下降,肾组织纤维化增多,丹参可通过消除氧自由基,提高抗氧化能力,减少组织纤维化来保护肾脏。

Objective: To explore the curative effect of renal peroxidative damage and fibrosis with Salvia Miltiorrhiza on left renal vein constriction rats to provide the experimental basis for the clinical therapy with drug of left renal vein entrapment syndrome.Method: SD rats were randomly divided into 3 groups,sham-operation group,model group,and salvia miltiorrhiza treating group.The rat model of left renal vein constriction was established by left renal vein partial deligation operation.Rats in treating group were intervened by salvia miltiorrhiza. After 6 weeks,all animals were killed. Renal tissue was homogenated to detect content of malonaldehyde(MDA) and activity of superoxide dismutase(SOD). Renal tissue section was used to detect TGF-β1 and PAI-1 expression.Results: Left renal tissue MDA content elevated,SOD activity reduced and TGF-β1,PAI-1 expression increased significantly in model group compared to those in sham-operation group.Treating with salvia miltiorrhiza could improve SOD activity,decrease MDA content and decrease TGF-β1 and PAI-1 expression.Conclusion: Generation of oxygen free radical in left renal vein constriction rats increased,ability of anti oxidisity decreased,and renal tissue fibrosis increased.Salvia miltiorrhiza has protective effect on left renal vein constriction rats,through blocking the oxygen free radical generation,elevating the ability of anti-oxidisity and decreasing tissue fibrosis.