高效液相色谱法检测牛初乳sIgA方法研究

Research on Determination of Bovine Colostrums sIgA by HPLC

建立高效液相色谱法测定牛初乳sIgA含量。采用TSK-G3000PWxl凝胶色谱柱以0.02mol/L,pH6.8的磷酸盐缓冲液为流动相,流速0.6mL/min,检测波长280nm。结果表明,牛初乳sIgA纯度为52.56%,回收率为74%。说明此方法方便、快捷、准确、可靠、重现性好,可用于牛初乳sIgA含量的检测。

Objective to establish HPLC method for the determination of bovine colostrums sIgA. sIgA was separated on TSK-G3000PWxl column with 0.02 mol/L, pH 6.8 PBS, and flow rate was 0.6 mL/min. sIgA was detected at UV 280 nm. Results showed that the purity and yield of sIgA were 52.56% and 74%, respectively. So the toothed is convenient, rapid, accurate, reliable and reproducible. It can be used for the detection of bovine colostrums sIgA.