自由基清除剂对PrP106-126诱导的分化PC12细胞存活率影响的研究

Study on free radical scavenger for survival rate of differentiated PC12 cells infected by prion protein 106-126 peptide

目的从细胞存活率角度观察探讨自由基清除剂—依达拉奉对PrP106-126诱导的分化PC12细胞损伤的保护作用,从而寻找更加有效的朊蛋白病的治疗方法。方法应用MTT法,体外观察不同浓度的依达拉奉对PrP106-126诱导的分化PC12细胞存活率改变的影响。结果不同浓度的依达拉奉(15、30、45、60μmol/L)对PrP106-126诱导的分化PC12细胞均有保护作用,随着浓度的增加,保护作用更加明显,呈量效和时效依赖关系。结论依达拉奉对PrP106-126诱导的分化PC12细胞具有抗氧化损伤保护作用,其作用可以通过提高感染PrP106-126后的分化PC12细胞的存活率而表现出来。

[objective] To explore the protection of free radical scavenger（edaravone）for the survival rate of differentiated PC12 cells infected by prion protein 106-126 peptide. [Methods] The protection inducing effect of edaravone on the differentiated PC12 cells infected by prion protein 106-126 peptide in vitro. All these changes in cells with 15, 30, 45, 60 μmol/L in edaravone were dynamically observed. They could be seen by MTT assay. [Resuits] The inverted phasecontrast microscope and a laser scan microscope results showed that edaravone could significantly protect the differentiated PC12 cells infected by prion protein 106-126 peptide when these cells were exposed to edaravone at 15, 30, 45, 60 μmol/L for 24 h, respectively. And it was in a dose dependent manner. [Conclusion] On the basis of this analysis, we suggest that edaravone may prevent the differentiated PC12 cells infected by prion protein 106-126 peptide from injured and weaken the injury of these cells.