功能失调性子宫出血患者宫内膜诱导型一氧化氮合酶和基质金属蛋白酶-9的表达及其意义

Expression of matrix metalloproteinase and inducible nitric oxide synthase in endometrial tissue

目的探讨诱导型一氧化氮合酶(INOS)和基质金属蛋白酶-9(MMP-9,又称明胶酶B)与功能失调性子宫出血(功血)的关系。方法分为病例组(30例)和对照组(10例),病例组标本来源为功血患者的子宫内膜组织,其中单纯性增生20例、复杂性增生10例。对照组标本为月经周期正常的子宫肌瘤患者行子宫切除术所取的子宫内膜组织。用RT-PCR技术检测各标本中诱导型一氧化氮合酶(INOS)及MMP-9的mRNA表达量。结果功血复杂性、单纯性增生组子宫内膜组织中INOS和MMP-9mRNA的表达量均显著高于正常对照组,(P<0.01)。结论INOS和MMP-9对功血的发生有联合作用,INOS及MMP-9的增高可证实功血与子宫内膜局部微环境的改变有关。

[Objective] To investigate the relationship of dysfunctional uterine bleeding （DUB） and the expression of INOS and matrix metalloproteinase-9（MMP-9, GelatinaseB）. [Methods] Forty samples were divided into two groups. The experiment group included 20 samples of simple hyperplasia endometrium and 10 samples of complex hyperplasia endometrium which origin from DUB patients. The control group including 10 samples originally from myoma patients whose menstrual cycle were normal and got hysterectomy. The expressions of INOS and MMP- 9 mRNA were studied with reverse transcriptase polymerase chain reaction（RT-PCR）. [Results] INOS and MMP-9 mRNA were significantly higher not only in simple hyperplasia DUB patients but also in complex hyperplasia DUB patients than those of proliferative stage of normal endometrium.（P 〈0.01）. [Conclusions] INOS and MMP-9 correlate well with the occurrence of DBU together. Higher expression of INOS and MMP-9 affect the uterine bleeding by influencing the local micro-circumstance of uterine.