鼠双微体-2mRNA及其蛋白表达与新疆维吾尔族妇女宫颈癌相关性研究

Expression of MDM2 mRNA and protein in cervical carcinoma in Xinjiang Uigur women

目的检测MDM2 mRNA及其蛋白在新疆维吾尔族妇女宫颈癌组织中的表达情况,探讨MDM2基因表达在宫颈癌发生发展中的作用。方法应用免疫组化方法检测79例宫颈癌、40例正常宫颈组织中MDM2蛋白表达;RT-PCR检测58例宫颈癌、29例正常宫颈组织中MDM2 mRNA表达。结果宫颈癌组织中MDM2蛋白表达率为39.2%(31/79),明显高于正常宫颈组织10.0%(4/40),差异有显著性(χ2=10.936,P=0.001)。宫颈癌及正常宫颈组织中MDM2 mRNA表达率分别为41.4%(24/58)、34.5%(10/29),两者差异无显著性(χ2=0.386,P=0.534)。对MDM2 mRNA阳性的宫颈癌及正常宫颈组织进行半定量检测,宫颈癌中MDM2 mRNA/β-actin mRNA平均灰度比值(1.432±0.509)与正常宫颈组织(0.938±0.283)相比,两者差异有显著性(t=2.852,P=0.008)。宫颈癌组织中MDM2蛋白表达及MDM2 mRNA表达之间具有相关性(χ2=6.801,P=0.009)。结论MDM2 mRNA与MDM2蛋白的高表达提示MDM2在维吾尔族宫颈癌发生中起重要作用。

[Objective] To investigate the expression and significance of MDM2 mRNA and protein in cervical carcinoma. [Methods] MDM2 protein of 79 cases of cervical carcinoma and 40 cases of normal cervical tissue were detected by immunohistochemistry envision methods, and MDM2 mRNA of 58 cases of carcinoma and 29 cases of normal tissue was detected using RT-PCR. [Results] The expression of MDM2 protein was detected in 31 of 79 （39.2%） in cervical carcinoma, and was significantly higher than that in normal group χ^2-=-10.936, P =0.001）. The overexpression of MDM2 mRNA was determined in cervical carcinoma by semiquantitive analysis. The average ratio of MDM2 mRNA/β-actin mRNA in cervical carcinoma was significantly higher than that in normal group （0.9379± 0.2830）. There was a significant correlation between MDM2 mRNA and MDM2 protein expression in cervical carcinoma （χ^2=6.801, P =0.009）. [Conclusion] The overexpression of MDM2 mRNA and MDM2 protein suggests that MDM2 may play an important role in eareinogengsis of Uigur cervical carcinoma.