意大利多元化砧木快速繁殖研究

Studies on Rapid Propagation of Italian Versatile Rootstock

以引进的意大利多元化砧木品种Penta的茎段为外植体,进行了快速繁殖研究。结果表明:合适的启动培养基为MS+BA 1.0 mg/L+IBA 0.3 mg/L;合适的增殖培养基为MS+6-BA 1.0 mg/L+IBA 0.3 mg/L+IAA 0.3 mg/L+NAA 0.2 mg/L;合适的生根培养基为1/2 MS(大)+IBA 1.0 mg/L;移栽以3条根以上的试管苗在蛭石∶土为5∶3的基质中移栽成活率最高。

Using the stem-segment with axilary buds as the explant, the rapid propagation of Italian Versatile Rootstock Penta was studied. The results showed： （1）The suitable medium for induction culture was MS＋BA 1.0 mg/L＋ IBA 0.3 mg/L; （2）The suitable multiplication medium was MS＋6-BA 1.0 mg/L ＋IBA 0.3 mg/L＋IAA 0.3 mg/L＋ NAA 0.2 mg/L; （3） The suitable rooting medium was 1/2MS＋IBA 1.0 mg/L; （4）Transition cultivating rate of tissue culture seedlings with more than 3 roots was best in the 3/5 mixed soil and vermiculite.