旱金莲的离体培养与快速繁殖

In Vitro Culture and Rapid Propagation of Tropaeolum majus

选取生长健壮、无病虫害带3～4叶的旱金莲侧枝,常规表面灭菌后,顶芽除去肉眼能见到的幼叶,侧枝分切成单芽茎段,接种于诱导培养基上,适宜的培养基为MS＋BA 0.5 mg·L^-1（单位下同）＋IAA 0.1～0.2。增殖培养基为MS＋BA 1.0＋IAA 0.1最合适,每3周作为一个继代周期,增殖倍数可达8～10倍。以1/2 MS＋NAA 0.2作为生根培养基,10 d生根,生根试管苗的移栽以混合基质草炭∶蛭石∶珍珠岩=3∶2∶1为好。

The strong and without disease and insect infection branch stems were carried as explants in vitro. In vitro culture and rapid propagation of Tropaeolum majus. The results showed that optimum media was MS＋ BA（0. 5 mg· L^-1） ＋IAA（0. 1-0. 2 mg· L^-1 ）, and the optimum subculture media were MS＋BA（1.0 mg · L^-1）＋IAA（0. 1 mg · L^-1 ）, the appropriate subculture cycles was 3 weeks, the proliferation rate was 8～10. Tropaeolum majus wouldl root in 10 day by using 1/2MS＋NAA （0.2 mg · L^-1 ） as rooting medium. The rooting plantlets transplanted into the mixed substrate with substrate containing 50% vermiculate, 33% pearlite, and 17% peat was suitable.