月季‘金银岛’的红花芽变品系的分析鉴定

Identification of Pink Sprout Mutation from 'Gold Island' Rose

芽变育种在月季新品种选育中至关重要。为了快速区分真正可遗传的芽变和环境因素引起的饰变,采用RAPD和ISSR对月季‘金银岛’的疑似芽变株系‘往日情怀’进行了标记分析,RAPD和ISSR引物都能扩增出差异多态性条带。结果表明:该芽变是由基因组变异引起的可遗传的稳定变异,是与花青素合成相关基因的突变。这为花色相关变异基因分离克隆以及‘往日情怀’芽变新品种申报奠定了基础。

Bud mutation is very important for new variety selection. In order to fast distinguish heritable mutation from the decoration mutation caused by environmental factor, this article used RAPD and ISSR to analysize ‘Gold Island＇and its suspected bud mutation Yesterday Love＇and polymorphic bands can be amplified with RAPD and ISSR primers. The result indicated this bud mutaion is the stable variation which resulted from the genornic variation. This work had laid the foundation for separation of the genes related to flower color as well as declaration of the new variety Yesterday Love＇.