薄层色谱扫描法监测风湿病患者血中解热镇痛药浓度

Identification of antipyretic analgesic in serum of rheumatisant by TLC scanning

目的建立风湿病患者血中解热镇痛药的血药浓度监测方法和解热镇痛药中毒的快速检验诊断方法。方法应用血清中解热镇痛药主要成分的提取和薄层色谱扫描定性和定量检测方法进行,血浆2ml,pH=6.0,乙醚10ml分2次萃取,20μl乙醇定容,点于HPGF254板上,环己烷∶三氯甲烷∶无水乙醇∶乙酸乙酯∶冰醋酸(9.0∶2.0∶1.5∶2.0∶1.0)展开5～7cm,薄层色谱扫描定性和定量检测。结果血清中对乙酰氨基酚的线性检测范围为0.2～40μg/ml,最小检出浓度为0.1μg/ml;塞来昔布的线性检测范围为1～35μg/ml,最小检出浓度为0.5μg/ml;氨基比林的线性检测范围为2～40μg/ml,最小检出浓度为1μg/ml;布洛芬的线性检测范围为5～50μg/ml,最小检出浓度为2.5μg/ml;双氯芬酸的线性检测范围为2～40μg/ml,最小检出浓度为1μg/ml。结论本法快速、简便,定性定量准确,可用于解热镇痛药中毒的快速诊断和风湿病患者血药浓度监测。

Objective To develop a test of antipyretic analgesic levels for serum titration and quick screening of overdose with these agents in rheumatics. Methods Each aliquot of blood sample （2.0 ml） was double-extracted with a total of 10 ml ether （pH=6.0）, metered to a constant volume with 20 μl ethanol, and transferred onto a HPGF254 plate used as the TLC plate. The plate was developed with a mixture containing cyclohexane, trichloromethane, anhydrous ethanol, acetic ether and glacial acetic acid （9.0：2.0：1.5：2.0：1.0 in parts） for 5 cm to 7cm. Qualitative and quantitative analyses of antipyretic analgesic were performed by TLC scanning. Results According to extraction curves, the linear ranges and minimum detectable limits were, respectively, 0.2-40μg/ml and 0.1μg/ml for paracetamol, 1-35 μg/ml and 0.5 μg/ml for celecoxib, 2-40 μg/ml and 1μg/ml for aminophenazone, 5-50 μg/ml and 2.5 μg/ml for ibuprofen, 2--40 μg/ml and 1 μg/ml for diclofenac. Conclusions TLC scanning provides a useful test for serum titration and a quick screen of overdose with antipyretic analgesics that appears simpler, sensitive and practical.