重组人生长激素联合骨髓间充质干细胞静脉移植对心力衰竭大鼠心功能的影响

Intravenous transplantation of bone marrow-derived mesenchymal stem cells combined with subcutaneous recombinant human growth hormone improves cardiac function in rats with adriamycin-induced cardiomyopathy and heart failure

目的探讨重组人生长激素(rGH)联合骨髓间充质干细胞(MSC)静脉移植对阿霉素诱导心肌病心力衰竭(心衰)大鼠的心功能影响。方法①分离纯化MSC进行体外培养,采用流式细胞仪检测其表面标记CD45,CD44和CD71。②将50只Wistar大鼠,选取阿霉素成功诱导的40只心肌病心衰模型,随机分成4组,心衰组,静脉移植组,rGH组,MSC+rGH组,rGH组为皮下注射,静脉移植组为尾静脉注射MSC,心衰组不给予任何干预措施。由彩色超声心动图和血流动力学检查监测心功能参数。结果发现体外培养的第2代MSC均表达CD44和CD71,不表达CD45。在静脉移植4周后,发现治疗组心功能指标(超声心动图和血流动力学指标)均有改善。静脉移植,rGH加静脉移植和rGH组3组之间心功能指标差异无统计学意义,但rGH加静脉移植较静脉移植和rGH两组心功能指标改善更明显,与心衰组相比心功能指标差异均有统计学意义。结论rGH联合MSC静脉移植对阿霉素诱导心肌病心衰大鼠的心功能有明显改善。

Objective To study the effects of bone marrow mesenchymal stem cells （MSCs） intravenous transplantation in combination with recombinant human growth hormone （rGH） on cardiac function of rats with adriamycininduced cardiomyopathy and heart failure. Methods （1） MSCs cells were isolated, cultured in vitro, and then purified. The cultured cells were detected for surface markers including CD45, CD44 and CD71, using a flow cytometer. （2） Of 50 Wistar rats, models of adriamycin-induced cardiomyopathy and heart failure were successfully generated in 40. These rats were randomized into one of 4 groups to receive MSCs intravenous transplantation via the caudal vein （MSC group）, subcutaneous rGH （rGH group）, combined use of IV MSC and rGH （MSC ＋ rGH group）, or nothing （heart failure group）. At 4 weeks, cardiac function was evaluated by echocardiography and hemodynamic measurement. Results Flow cytometric analysis showed that the second-passage MSC expressed CD44 and CD71 but not CD34. The cardiac function was improved in all treatment groups in terms of echocardiography and hemodynamic findings. Compared with heart failure group, MSC＋rGH group showed greater improvement in cardiac function than MSC group and rGH group. Conclusion MSC intravenous transplantation in combination with rGH provides significant be.nefits in rats with adriamycin-induced cardiomyopathy and heart failure.