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J-亚群禽白血病病毒中国广东野毒株的分离与鉴定 被引量:7

Isolation and Identification of Subgroup J Avian Leukosis Virus
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摘要 通过接种鸡胚成纤维细胞、间接荧光抗体试验(IFA)和聚合酶链式反应(PCR)从疑似J-亚群白血病的病鸡中,分离鉴定出J-亚群白血病病毒。抗J-亚群白血病病毒gp85单克隆抗体JE9的IFA试验和PCR试验均证明病鸡被J-亚群白血病病毒感染。 Subgroup JAvian Leukosis Virus was isolated and identified from a breeding farm with suspected sick chickens by inoculating the samples into embryo fibroblast, by indirect immunofluorescence assay (IFA) and PCR amplification technology. The results of IFA with ALV-J specific monoclonal antibody JE9 and PCR showed that the suspected sick chickens had been infected by ALV-J.
出处 《家畜生态学报》 2007年第5期89-91,95,共4页 Journal of Domestic Animal Ecology
关键词 禽白血病病毒 分离与鉴定 间接荧光抗体试验(IFA) 聚合酶链式反应(PCR) Avian Leukosis Virus isolation and identification immunofluorescence assays(IFA) polymerase chain reaction (PCR)
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参考文献15

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二级参考文献4

  • 1秦爱建.J-亚群鸡白血病病毒囊膜蛋白gp85基因及其产物的生物学和生物化学特性的鉴定:博士学位论文[M].扬州大学,1999..
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