塞来昔布对胶质瘤细胞线粒体膜电位的影响

Effect of celecoxib on mitochondrial membrane potential and apoptosis of glioma cells

目的观察塞来昔布(Celecoxib)降低U251脑胶质瘤细胞线粒体膜电位(MMP)并诱导其凋亡的相关规律。方法采用100μmol/L塞米昔布处理培养的U251细胞,并用流式细胞仪检测其MMP和细胞凋亡的变化。结果经塞来昔布处理后胶质瘤细胞MMP明显降低,并随时间延长而变化。单染实验中12 h和24 h处理组与对照组比较差异有统计学意义(P<0.01);12 h组与24 h组比较差异有统计学意义(P<0.01)。双染实验中Rh123-PI-细胞为细胞膜完整,MMP降低,即将发生凋亡的细胞,12 h处理组MMP明显降低,Rh123-PI-细胞明显增多(P<0.01);24 h处理组与对照组比较,MMP降低,Rh123-PI-细胞明显增多(P<0.01),但与12 h组比较,MMP降低,Rh123-PI-细胞明显增多(P<0.01)。结论塞米昔布能有效降低胶质瘤细胞线MMP并能诱导U251细胞发生凋亡,从而降低细胞氧化供能,使胶质瘤细胞处于缺氧状态。塞米昔布对胶质瘤细胞MMP的影响可能参与胶质瘤细胞凋亡。

Objective To investigate the apoptosis induced by celecoxib and explore the apoptotic mechanism in U251 glioma cells. Methods U251 g/ioma cells were treated with celecoxib. The rnitochondrial membrane potential and the apoptosis of glioma cells were measured by flow cytometry. Results The mitochondrial membrane potential was decreased and the apoptosis of glioma cells increased after treated with celecoxib. Conclusion Apoptosis could be induced by celecoxib in glioma cells. The decrease of the mitochondrial membrane potential in glioma cells treated with celecoxib took part in the process of cell aooptosis.