人胰岛素样生长因子-1基因表达载体的构建

Construction of human insulin-like growth factor 1 gene expression vector

目的构建人胰岛素样生长因子-1(hIGF-1)基因的表达载体。方法通过聚合酶链反应(PCR)方法从PCDB-MGF-1质粒中扩增出392 bp的靶片段,将其黏端克隆至pET-His表达质粒,然后应用限制性酶切、PCR扩增及序列测定等技术进行鉴定。结果成功构建了hIGF-1表达片段,酶切及测序证实读码框正确。结论成功克隆hIGF-1表达载体,为体外表达蛋白和行免疫治疗勃起功能障碍奠定基础。

Objective To construct expression plasmid of human insulin-growth factor-1. Methods Human insulin-like growth factor 1 （hIGF-1） gene fragment including 392 bp was obtained by PCR amplification from the PCDB-hIGF1 plasmid,cloned and identified by PCR, restrictive enzyme and sequencing. Results Haman insulin-like growth factor 1 expression vector was successfully cloned with correct open reading fragment. Conclusion hIGF-1 fragment was cloned into the pET-His expression vector.