脑伤宁对大鼠脑缺血再灌注损伤的保护作用

Protective effects of Naoshangning recipe on cerebral ischemia-reperfusion injury in rats

目的观察脑伤宁对大鼠脑缺血再灌注脑损伤的保护作用。方法凝闭大鼠双侧椎动脉4～5 h后,夹闭颈总动脉30 min,再灌注90 min,造成大鼠脑缺血再灌注损伤模型,观察脑伤宁对脑组织中生化指标及脑电图的影响。结果缺血再灌注造成脑组织严重损伤,脑组织中水、Na^＋、Ca^2＋含量及丙二醛（MDA）含量较对照组明显增高,而超氧化物歧化酶（SOD）活力较对照组降低,并伴有脑电图异常和脑组织结构病理性改变。夹闭颈总动脉前30 m in腹腔注射脑伤宁能降低脑组织中水、Na^＋、Ca^2＋含量及MDA含量,并提高SOD活力,对组织学检查及脑电图也有所改善。结论脑伤宁对脑缺血再灌注损伤具有保护作用。

Objective To study the protective effects of Naoshangning recipe on brain injury in rats, Methods The bilateral vertebral arteries of rats were occluded, and 4 ～ 5 h later, the carotid arteries of rats in the conscious condition were occluded for 30 min followed by reperfusion for 90 min. The effects of Naoshangning recipe on the contents of water, Na ^＋ , and Ca^2＋ in cortex, malondialdehyde （MDA） content and superoxide dismutase （SOD） activity in brain hemispheres, as well as the EEG activities were observed, Results 30 min ischemia and 90 min after reperfusion, the contents of water, Na ^＋ , Ca^2＋ , and MDA were increased, and the SOD activity was reduced with abnormal EEG activity and ischemic injury in the brain tissues, Naoshangning recipe decreased the elevated water, Na ^＋ , Ca^2＋ , and MDA contents, increased the SOD activity, reduced the ischemic injury of brain tissue, and promoted the recovery of EEG activities. Conclusion Naoshangning has protective effects on cerebral ischemia - reperfusion injury in rats.