慢性前列腺炎诱发大鼠IgA肾病模型血、尿IL-6的变化

Serum and urine IL-6 levels in rats with IgA nephropathy induced by chronic prostatitis

目的:探讨慢性前列腺炎(CP)诱发IgA肾病(IgAN)的机制。方法:将63只SD大鼠随机分为A组(单纯切肝组)、B组(单纯前列腺炎组)和C组(前列腺炎+切肝组)。比较3组大鼠尿蛋白、尿红细胞计数、血清肌酐和血、尿IL-6水平,肾组织病变程度,以及IgA、IgG、IgM、补体C3沉积情况。结果:3组大鼠之间尿蛋白、尿红细胞计数以及血清肌酐水平差异无显著性。第25周时,B、C组血、尿IL-6水平较A组高(均P<0.05),但B组与C组之间无明显差异。第25周时,C组大鼠IgA沉积的阳性率为72.7%(16/22),较A组的25.0%(3/12)和B组的23.1%(3/13)高(P<0.01)。C组22只大鼠16只系膜轻度增殖,系膜区以IgA沉积为主,75.0%伴有IgG和(或)IgM沉积;均无补体C3沉积;无明显血尿、蛋白尿。结论:CP在网状内皮系统功能低下可诱发大鼠IgAN;CP时,前列腺局部产生的IL-6水平增加,可能参与了CP诱发IgAN的这一过程。

Objective To explore the mechanism of lgA nephropathy induced by chronic prostatitis in rats. Methods Sixty-three SD rats were randomly divided into 3 groups, Group A ： liver lobectomy, Group B ： with prostatitis, and Group C： prostatitis ＋ liver lobectomy. The urine protein, urine red blood cells count, serums creatinine, serum and urine IL-6 were detected. The degree of glomerular mesangium proliferation and the deposition of IgA, IgG, IgM and C3 in each group were examined. Results There were no significant differences in urine protein, urine red blood cells count and serum creatinine among the three groups. At 25 week, the levels of sernm and urine IL-6 in group B and C were higher than those in Group A（P 〈 0.05） while there were no significant differences betweem group B and C, and the incidence of IgA deposition in Group C was 72.7%（ 16/22）, being higher than those in Group A （25.0%, 3/12） and Group B（23.1%, 3/13） （P 〈 0.01 ）. Mild glomerular mesangium proliferation with mainly IgA deposition was found in 16 out of 22 rats in Group C, while there were 75.0% with IgG deposition and/or IgM deposition. There were no C3 deposition, obvious urine protein and hematuria. Conclusions Chronic prostatitis can induce IgA nephropathy under the condition that the capability of IgA clearance have declined. The increased level of IL-6 in prostate may play an important role in the course of IgA nephropathy induced by chronic prostatitis.