正常人和不育者精子膜脂类过氧化反应和精子存活率的关系

Sperm viability and sperm peroxidation in infertile patients and male fertile volunteers

为分析精子膜脂类过氧化反应（ＬＰＯ）和精子存活率之间的关系，对１０例不育症患者和１０例正常人的精子分别与０，０．１，０．２，０．４，０．８和１．６ｍｍｏｌ／Ｌ阿魏酸（一种已知的抗氧化剂）温育，以调节精子膜ＬＰＯ率。精子膜ＬＰＯ的测定采用丙二醛（ＭＤＡ）硫代巴比妥酸比色法，精子存活率的测定采用曙红Ｙ染色法。用直线回归分析两个测定值之间的关系。结果不育症患者和正常人精子膜ＬＰＯ和精子存活率之间均存在明显的负相关。特别是不育症组，相关性更为显著，与正常组相比，其精子的ＭＤＡ含量更高，存活率更低。结果提示精子膜ＬＰＯ可能是造成精子存活率降低的主要原因之一，同时为精子功能损伤所致的男性不育症提示了一条可能的临床诊断和治疗途径。

Sperm lipid peroxidation (LPO)assayed by melondialdehyde(MDA) thiobarbituric acid method at 3h of incubation and study of sperm viability by eosin Y dye exclusion method at 1,3 and 6h of incubation after 0,0.1,0.2,0.4,0.8 or 1.6mmol/L ferulic acid(a known antioxidant used to regulated the lipid peroxidation rate of sperm membrane)being added were carried out for semen specimens collected from 10 infertile patients and 10 fertile volunteers.The relationship between sperm lipid peroxidation and sperm viability was examined by linear regression analysis.There was a close negative correlation between the two,especially in the infertile patients,the higher the MDA formation the lower the sperm viability.It was suggested that LPO might play a major role in the decrease of sperm viability and might serve as a parameter for the clinical diagnosis and treatment of male infertility due to sperm defect.