氧自由基、苯并[α]芘和佛波酯致NIH3T3细胞膜流动性改变

Effects of Superoxide Anion, B(α)P and TPA on the Membrane Fluidity of NIH3T3 Cells

利用标记膜脂及膜蛋白质的荧光标记物DPH和外N-（3芘）马来酰亚胺标记不同浓度细胞刺激物苯并[α]芘、佛波酯及氧自由基作用下的NIH3T3细胞，发现高剂量的刺激物对膜脂损伤显著，低剂量的刺激物虽对细胞膜脂未造成明显损伤，但已引起膜蛋白质构象改变。提示以上刺激物对膜结构的影响可能与影响细胞正常的调控导致细胞的无限生长有关，进而在致、促癌中发挥作用。

The membrane fluidity of NIH3T3 cells treated with low and high concentration of cell stimulatives (extracellular generated superoxide anion (O2), 12-O-tetra-decanoyl-phorbol-13-acetate (TPA ), and benzo (α)-pyrene [B(α)P]) was investigated by means of fluorescence la-bels l, 6-diphenyl-l, 3, 5 hexatriene (DPH) and N-(3-pyrene ) maleimide (N (3p) M). The high concentration of O2、TPA、B(α)P greatly increased the fluidity of cell membrane lipid domain. No changes of the florescence polarization of DPH was found in membrane lipid do-main treated with low concentration of O2, TPA, and B(α)P. However, decrease in the flu-oresence polar1zation of N(3p)M on the cell membrane protein domain damaged by low con-centration of cell stimulatives was observed, showing that these treatment could influence the con formation of membrane protein. The possible relationship between the changes of the conformation of membrane protein and the cell transformation and its carcinogenic machenisms were discussed.