补肾、化瘀及补肾化瘀法延缓免疫衰老的对比研究

A comparative study on the effects of tonifying kiney,removing blood stasis,and tonifying kidney combined with removing blood stasis on delaying immune senescence

目的从免疫衰老角度验证中医“正虚挟瘀是衰老的主要机理”的理论。方法给３组老年小鼠分别灌服补肾方（八味地黄汤）、化瘀方（丹坤汤）或补肾化瘀方（八味丹坤汤）４周后，用生物活性法检测脾淋巴细胞白细胞介素２（ＩＬ２）及腹腔巨噬细胞白细胞介素１（ＩＬ１）的产生量。结果老年小鼠ＩＬ１、ＩＬ２产生量均低于青年小鼠。给予补肾方和补肾化瘀方的老年小鼠ＩＬ１、ＩＬ２产生均有增加，且补肾化瘀优于补肾，而化瘀方则有抑制作用。结论扶正化瘀可能是延缓免疫衰老的理想途径。

Objective\ there are many viewpoints to expound the mechanism of aging in traditional Chinese medicine.We hold that the deficiency of Zhengqi accompanied with blood stasis is the mai nmechanism of aging,this hypothesis was testified from the aspect of immune senescence in this exper i ment. Methods\ Three groups of old mice were medicated with tonifying kidney decoction(Bawei dihuangtang),removing blood stasis decoction(Dankuntang)or tonifying kidney combined with remov ing blood stasis decoction(Baweidankuntang)separately.After medication for 4 weeks,the producti onof interleukin 1(IL 1)by peritoneal macrophages and interleukin 2(IL 2) by splenic lymphocytes were assayed with the method of biological activity analysis.\ \ Results\ Both IL 1and IL 2decreased in old mice when compared with those of young controls.The production of IL 1and IL 2was restored in old mice treated with tonifying kidney decoction and tonifying kidney combined with removing blood stasis decoction and the effect of tonifying kidney combined with removing blood stasis decocti on was more than that of tonifying kidney decoction alone.However,in old mice treated with removing blood stasis decoction,the production of IL 1and IL 2was suppressed when compared with that of the old control. Conclusions\ Strengthening Zhengqi accompanied with removing blood stasis is an ide almethod for delaying immune senescence.