冬虫夏草对抗环磷酰胺致小鼠生精障碍的作用

Effects of cordyceps on the spermatogenic functional lesion induced by cyclophosphamide.

目的冬虫夏草(cordyceps)具有清除自由基,抗氧化的作用。本实验研究冬虫夏草对抗环磷酰胺致小鼠生精功能损害的作用。方法随机将小鼠分成3组:正常对照组、模型对照组、冬虫夏草治疗组。模型对照组和冬虫夏草治疗组小鼠按照40mg/kg.d腹腔注射环磷酰胺(cp)连续5天,同时冬虫夏草治疗组小鼠每天给予冬虫夏草混悬液300mg/kg.d灌胃;正常对照组小鼠及模型对照组灌胃等量生理盐水。各组均连续处理28天。末次给药24h后,处死全部小鼠,观察小鼠附睾精子密度,活力,活动率,睾丸组织匀浆后测定超氧化物歧化酶(superoxide d ismutas,SOD),谷胱甘肽过氧化物酶(glutath ione Peroxidase,GSH-Px),丙二醛(m alon iealdehvde,MDA)含量。结果与模型对照组相比,治疗组精子密度,活力,活动率,睾丸组织SOD与GSH-Px含量等均明显升高(P<0.05,P<0.01),精子畸形率和MDA含量有明显降低(P<0.05,P<0.01)。结论冬虫夏草可对抗环磷酰胺所致的小鼠生精功能的损害,其作用机制可能与冬虫夏草可清除氧自由基,抗氧化作用有关。

Objective： Cordyeeps can clean free radical and has anti -oxidation aetlon, to observe the protective effects of eordyeeps on the damage of mouse spermatogenes induced by eyelpphosphamide. Methods： The mlee were randomly divided into three groups ： the normal group, the control group, the erdyeeps treatment group. Cordyeeps treatment group and control group in accordance with 40mg/kg · d by intraperitoneal injection of eyelophosphamide in five days, while the erdyeeps treatment group was gavaged with eordyeeps suspension at 300mg/kg · d and the normal group were gavaged with normal saline through mouth. The treatment period for all groups was 28 days. All mice were sentenced to death 24h after the last treatment. Testis homogenate were prepared and the level of SOD, GSH - Px, MDA were measured respectively. Sperm density, sperm motility, sperm motility and sperm abnormality rate of epldldymlds were observed. Results ： In the eordyeeps treatment group, the level of SOD, GSH - Px and sperm density, sperm motilitywere increase significantly （ P 〈 0. 05, P 〈 0. 01 ）, the level of MDA and sperm abnormality rate were decreased significantly （ P 〈 0. 05, P 〈 0.01 ）. Conclusion： Cordyeeps has a significantly protective effect on spermatogenic damage by eyelophosphamide, the action of eordyeeps maybe have relationship with its scavenging free radical and anti - oxidation effect.