内异康复栓对子宫内膜异位症大鼠异位和在位内膜雌、孕激素受体的影响

Influence of Neyikangfu Suppository on Estrogen Receptors and Progesterone Receptors in Ectopic and Eutopic Endometrium of Endometriosis Model Rats

目的观察内异康复栓直肠给药对子宫内膜异位症大鼠异位和在位内膜雌激素受体(ER)、孕激素受体(PR)表达的不同影响,探讨其作用机理。方法将子宫内膜异位症大鼠随机分为内异康复栓高、中剂量组及丹那唑组、丹莪妇康煎膏组、模型组、空白组,采用免疫组化SP法检测ER、PR的表达。结果内异康复栓高、中剂量组能显著降低异位内膜ER、PR的表达(P<0.05),并对在位内膜下降的ER、PR水平有上调作用(P>0.05)。结论内异康复栓可能通过抑制异位内膜细胞内ER、PR的合成而发挥治疗作用,且对在位内膜异常的ER、PR表达有一定的调节作用。

Objective To explore the different influence of Neyikangfu suppository （NYKFS） on ER and PR in ectopic and eutopic endometrium of endometriosis model rats by treatment of rectal administration. Methods Healthy and sexual matured female SD rats were taken as the researching objects and endometriosis model were achieved by autologous operation transplantation. Six groups were randomly formed and which were high-dose and middle-dose groups of NYKFS, Danazol capsule group and Danefukangjiangao （DEFKJG） group, model group and normal control group. Expressions of ER and PR in ectopic and eutopic endometrium and endometrium of normal group was measured by immunohistochemical SP staining method. Results High-dose and middle-dose groups of NYKFS could obviously lower the expression of ER and PR in ectopic endometrium of endometriosis model rats. High-dose and middle-dose groups of NYKFS could increase the expression of ER and PR in eutopic endometrium to certain extent （P〉0.05）. Conclusions NYKFS can inhibit the synthesis of ER and PR in cells of ectopic endometrium to treat endometriosis and have certain accommodation on abnormal expression of ER and PR in eutopic endometrium.