补肾中药对增龄大鼠成骨细胞Cbfα_1表达的影响

Effects of Bushen Chinese Medicine on Expression of Cbfα1 in Aging Osteoblast of Rat

目的观察核心结合因子α1(Cbfα1)在增龄成骨细胞中的表达,探讨补肾中药促进骨形成的新机制。方法来源于不同年龄的大鼠成骨细胞被分离,采用组织块翻转方法培养。通过倒置显微镜观察细胞形态,矿化结节染色对成骨细胞加以鉴定。采用RT-PCR和免疫组化方法检测成骨细胞Cbfα1的表达。结果RT-PCR和免疫组化结果表明,在增龄成骨细胞中,固本壮骨胶囊、金匮肾气丸和西药萌格旺可上调不同龄大鼠成骨细胞Cbfα1的表达,知柏地黄丸则下调Cbfα1的表达,补肾益精方对于不同龄大鼠成骨细胞Cbfα1表达的影响存在着区别。结论补肾中药中的补阳中药具有促进骨形成的作用。

Objective To observe the expression of core-binding factor α1（Cbfα1） in aging osteoblast, and try to provide new mechanisms of Chinese medicine on bone formation. Methods Rat osteoblast from different age donors （6 months and 16 months） were isolated and cultured by the tissue-fragment- migrating-growth method. The biological characteristic was observed by phase-contrast microscope and identified with mineralizing nodule staining. The expression of Cbfα1 was examined by RT-PCR and immunohistochemistry. Results RT-PCR and immunohistochemistry showed that the expression of Cbfα1 was upregulated by Gubenzhuanggu and Jinkuishenqi, and downregulated by Zhibaidihuang in aging osteoblast, but the effect was different on the Cbfα1 expression in Bushenyijing. Conclusion Buyang Chinese medicine among Bushen Chinese medicine promoted bone formation.