慢性丙肝RIBA与RIA-2检测抗-HCV的价值

Diagnostic value of RIBA and RIA 2 in detection of anti HCV in chronic hepatitis C

目的探讨重组免疫印迹试验（ＲＩＢＡ）在慢性ＨＣＶ感染中的应用价值．方法１９９３年１月～１９９５年３月采用含ＨＣＶ不同编码区三种重组抗原（Ｃ２２，Ｃ３３ｃ，Ｃ１００３）的ＲＩＢＡ，对８５例慢性非甲非乙型肝炎（ＮＡＮＢＨ）患者血清进行检测并将其结果与第二代放免法（ＲＩＡ２）检测抗ＨＣＶ及逆转录多聚酶链反应（ＲＴＰＣＲ）检测ＨＣＶＲＮＡ结果进行比较分析．结果慢性ＮＡＮＢＨ患者血清ＲＩＢＡ阳性（７８８％，６７／８５）结果与ＨＣＶＲＮＡ阳性（７５３％，６４／８５）结果之间有良好相关性和高的符合率；ＲＩＢＡ可检出ＲＩＡ２阴性的ＨＣＶ感染者；单项抗体阳性时仅见一定比例的单项抗Ｃ２２或抗Ｃ３３ｃ阳性血清可检出ＨＣＶＲＮＡ，而单一抗Ｃ１００３阳性者ＨＣＶＲＮＡ均阴性．结论ＲＩＢＡ可提高慢性ＨＣＶ感染的诊断率，ＲＩＢＡ阳性为ＨＣＶ感染及病毒血症存在的标志，可作为ＲＩＡ２检测抗ＨＣＶ的确证实验．

AIM To explore the diagnostic value of recombinant immunoblot assay (RIBA) in chronic HCV infection. METHODS RIBA including three recombinant HCV antigen (C 22 , C 33c and C 100 3 ) was developed in our lab, and serum samples from 85 patients with chronic non A non B hepatitis (NANBH) were investigated. The results of RIBA were compared with those of RIA 2 (second generation radioimmunoassay for anti HCV) and serum HCV RNA detected by RT PCR (reverse transcription polymerase chain reaction). RESULTS There was a close relationship and a higher agreement rate between the results of RIBA and HCV RNA in patients with chronic NANBH. RIA 2 negative HCV infection can be detected by RIBA; HCV RNA was detected in a certain proportion of “single anti C 22 positive or single anti C 100-3 positive” patients. HCV RNA was negative in all single anti C 100-3 positive patients. CONCLUSION RIBA can increase diagnostic rate of chronic HCV infection.