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慢性丙肝RIBA与RIA-2检测抗-HCV的价值 被引量:1

Diagnostic value of RIBA and RIA 2 in detection of anti HCV in chronic hepatitis C
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摘要 目的探讨重组免疫印迹试验(RIBA)在慢性HCV感染中的应用价值.方法1993年1月~1995年3月采用含HCV不同编码区三种重组抗原(C22,C33c,C1003)的RIBA,对85例慢性非甲非乙型肝炎(NANBH)患者血清进行检测并将其结果与第二代放免法(RIA2)检测抗HCV及逆转录多聚酶链反应(RTPCR)检测HCVRNA结果进行比较分析.结果慢性NANBH患者血清RIBA阳性(788%,67/85)结果与HCVRNA阳性(753%,64/85)结果之间有良好相关性和高的符合率;RIBA可检出RIA2阴性的HCV感染者;单项抗体阳性时仅见一定比例的单项抗C22或抗C33c阳性血清可检出HCVRNA,而单一抗C1003阳性者HCVRNA均阴性.结论RIBA可提高慢性HCV感染的诊断率,RIBA阳性为HCV感染及病毒血症存在的标志,可作为RIA2检测抗HCV的确证实验. AIM To explore the diagnostic value of recombinant immunoblot assay (RIBA) in chronic HCV infection. METHODS RIBA including three recombinant HCV antigen (C 22 , C 33c and C 100 3 ) was developed in our lab, and serum samples from 85 patients with chronic non A non B hepatitis (NANBH) were investigated. The results of RIBA were compared with those of RIA 2 (second generation radioimmunoassay for anti HCV) and serum HCV RNA detected by RT PCR (reverse transcription polymerase chain reaction). RESULTS There was a close relationship and a higher agreement rate between the results of RIBA and HCV RNA in patients with chronic NANBH. RIA 2 negative HCV infection can be detected by RIBA; HCV RNA was detected in a certain proportion of “single anti C 22 positive or single anti C 100-3 positive” patients. HCV RNA was negative in all single anti C 100-3 positive patients. CONCLUSION RIBA can increase diagnostic rate of chronic HCV infection.
出处 《新消化病学杂志》 1997年第7期444-445,共2页
关键词 丙型肝炎 诊断 放射免疫测定 RIBA RIA-2 Hepatitis C/diagnosis\ \ RNA, viral/analysis\ \ Polymerase chain reaction\ \ Radio immoassay
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  • 1王育强,任仲轩.酶免疫分析试剂的临床使用价值[J].解放军医学杂志,1996,21(1):53-54. 被引量:1
  • 2Mimms L,Vallari D,Ducharme L,et al.Specificity of anti-HCV ELISA assessed by reactivity to three immunodominant HCV regions. The Lancet . 1990
  • 3Theilmann L,Blazek M,Goeser T,et al.False-positive anti-HCV test in rheumatoid arthritis. The Lancet . 1990
  • 4Marcellin P,Martinot-Peignoux M,Boyer N,et al.Second generation(RIBA) test in diagnosis of chronic hepatitis C. The Lancet . 1991
  • 5Leon A,Canon R,Elia M,et al.Second generation RIBA to confirm diagnosis of HCV infection. The Lancet . 1991
  • 6汪涛,杜平.丙型肝炎病毒感染特异性检测的研究进展[J].国外医学(临床生物化学与检验学分册),1993,14(1):2-5. 被引量:3

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