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Isolation of a novel polyvalent virulent bacteriophage of E.coli

Isolation of a novel polyvalent virulent bacteriophage of E.coli
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摘要 Objective:To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission electron microscopy (TEM), genome extraction and nucleases test were used to isolate bacteriophages. Then its morphology and characteristics were examined. Results: A novel bacteriophage XY-1 was isolated from a sewage pond in a hospital. It infected and killed 6 E.coli reference strains. The phage had a round head (diameter 40-50 nm), no tail and the genome was ssRNA of approximately 5.0 kb. It was able to reduce E.coli to an extent of 44.63% to 67.00% when being added into the samples of different raw sewage water, depending on the contact time, the temperature and the phage dose. Conclusion: From the morphology typical and nucleotide characteristics (RNA) of the genome of phage, phage XY-1 appears to be closely related to phage f2. It may have some effects for the control of E.coli in sewage water. Objective:To isolate virulent bacteriophage from environment samples and explore the potential way of decontaminating the environmental wastewater. Methods: The standard plaque assay, negative staining transmission electron microscopy (TEM), genome extraction and nucleases test were used to isolate bacteriophages. Then its morphology and characteristics were examined. Results: A novel bacteriophage XY-1 was isolated from a sewage pond in a hospital. It infected and killed 6 E.coli reference strains. The phage had a round head (diameter 40-50 nm), no tail and the genome was ssRNA of approximately 5.0 kb. It was able to reduce E.coli to an extent of 44.63% to 67.00% when being added into the samples of different raw sewage water, depending on the contact time, the temperature and the phage dose. Conclusion: From the morphology typical and nucleotide characteristics (RNA) of the genome of phage, phage XY-1 appears to be closely related to phage f2. It may have some effects for the control of E.coli in sewage water.
出处 《Journal of Medical Colleges of PLA(China)》 CAS 2007年第5期261-267,共7页 中国人民解放军军医大学学报(英文版)
基金 Supported by the National Natural Science Foundation ofChina (No. 30571637) the Scientific Research Project ofthe Tenth Five-year Plan of PLA (No. 01MA170)
关键词 Escherichia coli polyvalent bacteriophage sewage water 大肠杆菌 噬菌体 污水 症状
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