甘露寡糖对小鼠肝脏中自由基的清除及抗氧化酶活性的影响

EFFECT OF MANNOSE-OLIGOSACCHARIDES ON SCAVENGING FREE REDICALS AND THE ACTIVITIES OF ANTIOXIDANT ENZYMES IN LIVER OF RATS

目的:研究微波合成的甘露寡糖(MOS)在体外清除羟自由基(·OH)及小鼠肝脏清除自由基和抗氧化酶活性。方法:以甘露糖为原料合成甘露寡糖。体外:测定其对于羟自由基(·OH)的清除率。体内:昆明种雄性小鼠(体重:20±2g)36只,按体重随机分为6组,每组6只,每日灌胃一次,分别为正常对照组(2g/kg生理盐水)、葡萄糖组(2g/kg葡萄糖)、甘露糖组(2g/kg甘露糖),MOS加葡萄糖组(MOS1g+葡萄糖)1g/kg,低浓度MOS组(MOS2g/kg),高浓度MOS组(MOS5g/kg),灌胃14d后,无菌取肝脏组织待测。结果:补充1～2g/kg的MOS可提高小鼠肝脏组织SOD、CAT、GSH-Px活性,提高GSH水平,提高肝脏组织总抗氧化能力及对·OH的清除率,降低MDA含量及ROS水平。结论:MOS体外可有效清除羟自由基(·OH);小鼠适量添加MOS有益于提高肝脏组织的抗氧化能力,保护肝脏免受各种氧化应激和损伤。

Objective： To evaluate the activities of mannose-oligosaccharides（MOS） on scavenging free radicals and the antioxidant enzymes in liver. Methods： We synthesized mannose-oligosaccharides and studied its effect on scavenging of hydroxyl radical in vitro. Thirty-six male Kunming mice （weight： 20±2g） were randomly divided into 6 groups： normal control group （2g/kg sodium chloride）, glucose group （2g/kg glucose）, rnannose group （2g/kg mannose）, glucose and MOS group （lg/kg glucose and lg/kg MOS）, low dose MOS group （2g/kg MOS） and high dose MOS group（5g/kg MOS）. After i.g administration of 0.2ml/d for 14d, livers were sampled in germfree condition. Results： The activities of antioxidant enzymes including superoxide dismutase（SOD）, catalase（CAT）, glutathione peroxidase （GSH-Px）, the level of glutathione （GSH）, total anti-oxidative ability and the scavenging rate of hydroxyl radical were increased, and the malondialdehyde （MDA） and the level of reactive oxygen species （ROS） were decreased with MOS supplement. Conclusion： MOS can scavenge the hydroxyl radical efficiently in vitro and may increase the anti-oxidation ability of liver in vivo. So MOS may have effects to protect livers from oxidative stress and damage.