肾炎2号对IgA肾病大鼠α-平滑肌肌动蛋白表达的实验研究

Experimental Research on NO.2 Prescription for Nephritis on the Expression of α-smooth Muscle Actin(α-SMA) in the Glomerular of IgA Nephropathy

目的：探讨肾炎2号对IrA肾病大鼠肾小球内α-平滑肌肌动蛋白（α-SMA）表达的影响。方法：使用口服和尾静脉注射牛血清白蛋白（BSA）的方法进行IrA肾病模型的制作，治疗组、对照组造模同时分别给予肾炎2号（15g／kg）和福辛普利（4．17mg／kg）至12周末。观察尿常规、24h尿蛋白定量、肾组织的病理改变及肾小球内α-平滑肌肌动蛋白（α-SMA）的表达。结果：①第7～12周末模型组的24h尿蛋白定量明显高于正常组、治疗组与对照组（P〈O，01）。②与模型组比较，治疗组大鼠肾小球内α-SMA的表达减弱（P〈0．01），但治疗组、对照组相比无明显统计学上的差异（P〉0，05）。结论：肾炎2号具有减轻肾小球内α-SMA的表达的作用。

Objective ： To investigate the effect of NO. 2 prescription for nephritis on the expression of α - smooth muscle actin （α -SMA） in the glomcrular of IgA ncphropathy rat. Methods ：Replicating IgA ncphropathy by taking orally acidified water （ containing 0. 1% bovine serum albumin - BSA） every other day and time - controlled vein injecting PBS buffer （containing 1% bovine serum albumin） after six weeks （one time every day, continuous 3 days ） , it takes 12 weeks. The treated group,cntrolled group are oralled decoction of NO, 2 prescription （15g/kg） and Fosinopril （4.17mg/ kg） respectively at the beg/nning of replicating model （ one time per day） ; Examine urine analysis and 24 hour urine pro- teinuria（24h Upm）amount from the end of 7th to 12th week ,Finnally observe the kidney tissue histopathology and the ex- pression of α- smooth muscle actin（α -SMA） in the glomemlar. Results ：①It is found 24h Upm in M group is higher obviously than the other groups from the end of 7th to 12th week （ P 〈 0.01 ） ; ②Kidney immunohistochemistry shows expression of α - SMA in the glomerulus in treated group is decreased significantly than the model （ P 〈 0.01 ）, but it has no difference between the treated group and controlled group （ P 〉 0.05）. Conclusion ： It shows NO. 2 prescription has the function of reducing the expression of α - SMA in the glomemlus.