摘要
运用体外实验、对Vc／FeSO_4。诱发的微粒体脂质过氧化（LP）发生条件及反应历程进行了探讨。结果表明：在37℃水浴30min，微粒体蛋白浓度为1.0mg／ml，4μmol／LFeSO_4.pH值7．4的0．1mol／LTris－HCl缓冲浪的条件下，Vc低浓度（0.25mmol/L以下）时可刺激LP，但高浓度的Vc（＞0.25mmol/L）则抑制LP，其中Vc为0．05mmol/L时．微粒体中MDA含量于反应后迅速上升，30min达到高峰.以后趋于平稳。揭示：一定比例的Vc／FeSO_4可有效地激发微粒体LP。本研究初步建立了微粒体LP模型。
The condition and the time course of lipid peroxidation (LP)induced by Vc/FeSO4 in rat hepatic microsomes in vitro was studied. The results revealed that when microsomes (1. 0mg protein/ml) were incubated with 4umol/LFeSO4at 37 C for 30 min in pH7. 4,O. lmol/L, Tris-HCl buffer, Vc, at lowconcentrations(<0.25mmol/L ) stimulated LP.but at higher concentration (>0.25mmol/L),Vc inhibited LP. The content of Malonaldehyde (MDA) rised rapidly after addition of 0.05mmol/L Vc and 4umol/L, FeSO_4 O4, reached maximal levels in 30min, very litle additional LP occured over the next two hours. The rcsullt suggested that Ve/FeSO_4 on the appropriate scale could induced ellectively microsomal LP. A model induced by Vc/FeSO4 was founded preliminarily in our experiment.
出处
《中国卫生检验杂志》
CAS
1997年第2期73-75,共3页
Chinese Journal of Health Laboratory Technology
