期刊文献+

1,25二羟基维生素D_3对大鼠脾调节性T细胞及其特异性转录因子表达的影响 被引量:2

The effect of 1,25(OH)_2 VitD_3 on CD4^+ CD25^+ regulatory T cells and transcription factor Foxp3 in rat spleen
下载PDF
导出
摘要 目的:探讨用1,25二羟基维生素D3[1,25(OH)2VitD3]预干预对大鼠辅助性T细胞(Th1)免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3基因表达的影响。方法:采用近交系大鼠,随机分为对照组、脂多糖(LPS)组和VitD3组。分别给予对照组和LPS组赋形剂,VitD3组用1,25(OH)2VitD3(0.25μg/只.d-1),连续灌胃14 d。第15天LPS组和VitD3组腹腔注射致死剂量LPS(10 mg/kg)。6 h后处死,用流式细胞仪检测脾CD4+CD25+调节性T细胞比例,用Realtime RT-PCR检测脾中Foxp3 mRNA的表达。结果:1,25(OH)2VitD3明显上调大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞的比例及其特异性转录因子Foxp3 mRNA的表达。结论:1,25(OH)2VitD3预干预对大鼠Th1免疫应答中脾CD4+CD25+调节性T细胞及其特异性转录因子Foxp3的基因表达有显著影响。1,25(OH)2VitD3有可能通过促进CD4+CD25+调节性T细胞的发育,发挥其免疫调节作用。 Objective: To investigate the effect of 1,25(OH)2 VitD3 on CD4^+ , CD25^+ regulatory T cells and its specific transcription factor Foxp3 in rat spleen in Th1 immune reactioin, Methods: Lewis rats were randomly divided into 3 groups. VitD3 group was fed with 1,25 (OH)2 VitD3 (0-25 p,g per rat a day) for 14 days. The control group and LPS group administered the isotonic NaCl in the same volume for the same long time. LPS (10 mg/kg) was administered intraperitoneally in LPS group and VitD3 group on the 15th day, and then the animals were sacrificed 6 hours later. The percentage of CD^+ CD25^+ regulatory T cells in lymphomonocytes in rat spleen was analyzed by Flow cytometry. The expression of Foxp3 gene in rat spleen was detected by Realtime RT-PCR. Results: 1,25 (OH)2 VitD3 increased the percentage of CD^4+ CD^25+ regulatory T cells in lymphomonocytes in rat spleen significantly. 1,25 (OH) 2 VitD3 also up regulated the expression of Foxp3 gene markedly. Conclusion: 1,25(OH)2 VitD3 has a great influence on CD^+ CD25^+ regulatory T cells and its specific transcription factor Foxp3. 1,25 (OH)2 VitD3 may enhance the development and function of CD4^+ CD25^+ regulatory T cells either directly or indirectly.
出处 《肠外与肠内营养》 CAS 2007年第3期129-131,136,共4页 Parenteral & Enteral Nutrition
基金 国家重点基础研究发展规划项目资助(2003CB515502)
关键词 1 25二羟基维生素D3 CD4+CD25+调节性T细胞 特异性转录因子Foxp3 1, 25-Dihydroxyvitamin D3 CD^+ CD25^+ regulatory T cell Foxp3
  • 引文网络
  • 相关文献

参考文献9

  • 1Ewa Marcinkowska.A run for a membrane vitamin D receptor[J].Biol Signals Recept,2001,10(6):341-349.
  • 2Hector F.Deluca and Margherita T.Cantorna.Vitamin D:its role and uses in immunology[J].FASEB J,2001,15(14):2579-2585.
  • 3Hullett DA,Cantorna MT,Redaelli C,et al.Prolongation of allograft survival by 1,25-dihydroxyvitamin D3[J].Transplantation,1998,66 (7):824-828.
  • 4Jason D Fontenot,Alexander Y Rudensky.A well adapted regulatory contrivance:regulatory T cell development and the forehead family transcription factor Foxp3[J].Nat Immunol,2005,6 (4):331-337.
  • 5Shohei Hori,Shimon Sakaguchi.Foxp3:a critical regulator of the development and function of regulatory T cells[J].Microbes and Infection,2004,6(8):745-751.
  • 6Fontenote JD,Gavin MA,Rudensky AY.Foxp3 programs the development and function of CD4+CD25+ regulatory T cells[J].Nat.Immunol,2003,4 (4):330-336.
  • 7Sakguchi S,Sakguchi N,Asano M,et al.Immunologic self-tolerance maintained by activated T cells expressing IL-2 receptor alpha-chains(CD25).Breakdown of a single mechanism of self-tolerance causes various autoimmune disease[J].J Immunol,1995,155(1):1151-1164.
  • 8Hori S,Nomura T,Sakaguchi S.Control of regulatory T cell development by the transcription factor Foxp3[J].Science,2003,299(5609):1057-1061.
  • 9Silvia G,Mara C,Susana A,et al.Regulatory T cells induced by 1α,25-dihydroxyvitamin D3 and mycophenolate mofetil treatment mediate transplantation tolerance[J].J Immunol,2001,167(4):1945-1953.

同被引文献12

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部