龈沟液中的炎症调节因子——白细胞介素8

Interleukin 8, a regulator of inflammation in gingival crevicular fluid

为研究白细胞介素８（ｉｎｔｅｒｌｅｕｋｉｎ－８，ＩＬ－８）在牙周炎中的作用，作者应用ＥＬＩＳＡ法检测了５４例（１０５个牙）成人牙周炎和２４名（５４个牙）健康对照者龈沟液中ＩＬ－８的水平。结果显示：龈沟液中ＩＬ－８的浓度低于３０μｇ／Ｌ时，ＩＬ－８与龈沟出血指数呈正相关（Ｐ＜０．０１）。龈沟液中ＩＬ－８的浓度高于３０μｇ／Ｌ时，ＩＬ－８与出血指数和探诊深度呈负相关（Ｐ值均小于０．０１）。９１％的牙周炎患牙龈沟液中ＩＬ－８低于３０μｇ／Ｌ。结果提示：ＩＬ－８可能具有抗炎和致炎的双重调节作用；其抗炎和致炎作用的分界值约为３０μｇ／Ｌ。高浓度的ＩＬ－８具有抗炎作用，低浓度的ＩＬ－８具有致炎作用。牙周炎时，龈沟液中的ＩＬ－８主要起致炎作用。

The role of interleuking 8 (IL 8), a neutrophil attracting and activating cytokine, was investigated in gingival crevicular fluid (GCF). ELISA was used to detect the levels of IL 8 in GCF collected from 54 adult periodontitis (AP) patients (105 teeth) and 24 healthy subjects (54 teeth). The results showed that ①The role of IL 8 was dependent upon the concentration of IL 8 in GCF. IL 8, in the low concentration (≤ 30 μg/L), was positively correlated with bleeding index ( r =0.36, P < 0.01). While in the high concentration (>30 μg/L), was negatively correlated with bleeding index and probing depth ( r =-0.54 and r =-0.65 respectively, P <0.01). In the majority of periodontitis sites (91%), the concentration of IL 8 in GCF were lower than 30 μl/L. IL 8 most likely acted as pro inflammatory factor in these teeth. ②IL 8 was a two way regulator of inflammation, pro inflammation and anti inflammation. The threshold of IL 8 between inducing and suppressing inflammation was approximately 30 μg/L. Within the confined range, IL 8 concentration greater than 30 μg/L could be inflammation suppressive, while a less than 30 μg/L concentration of IL 8 might become inflammation inducing.