β-catenin、c-myc在结直肠肿瘤中的表达及其意义

Expression of β-catenin and c-myc in human colorectal adenocarcinoma

目的:探讨β-catenin、c-myc的表达在结直肠肿瘤发生发展中的作用及其与临床病理参数之间的关系。方法:应用免疫组织化学SP法检测β-catenin及c-myc在结直肠正常黏膜、结直肠腺瘤和结直肠腺癌中的表达。结果:(1)β-catenin在正常黏膜中为胞膜表达,腺瘤与腺癌组呈胞浆或核异位表达,异位表达率为10.8%、75.0%、98.2%,3组之间有显著差异(P<0.01)。(2)正常黏膜、结直肠腺瘤和结直肠腺癌中c-myc的表达率为10.8%、40.6%、70.2%。3组之间有显著差异(P<0.01)。(3)结直肠腺癌中β-catenin的异常表达随着肿瘤大小、组织学分级、Dukes分期的改变呈逐渐增高趋势,有淋巴结转移者β-catenin阳性表达率(100.0%)高于无淋巴结转移者(96.4%),但无统计学意义(P>0.05)。结论:β-catenin的异常表达及c-myc的过表达与结直肠肿瘤的发生发展有关,β-catenin的异常表达可能与结直肠腺癌的恶性行为有关。

Objective： To investigate the expression of β-catenin and c-myc in colorectal carcinomas and their relation to clinical pathological parameters. Methods： The expression of β-catenin and c-myc protein was detemined with immunohistochemical SP method in the normal mucosas, the adenomas and the adenocarcinomas. Results： （1） β-catenin expression was detected on the cell membrane in normal colorectal mucosa. Cytoplasmic and nuclear expression was detected in the colorectal adenomas and adenocarcinomas. The ab- errant expression rates of β-catenin were 10.8%, 75.0% and 98.2%. There were significant difference among these three groups（ P 〈0.01）. （2） c-myc was expressed in 10.8% of the normal mucosas, 40.6% of the adenomas and 70.2% of the adenocarcinomas. There were significant difference among these three groups（ P 〈0.01）. （3） In carcinoma of intestine the bigger the tumors size and the higher grade were, the higher the aberrant expression of β-catenin was. Tumors with regional nodal metastasis showed higher abnormal staining rate （100.0%） than those without nodal involvement （96.4%）. But there was no significant difference（ P 〉0.05）. Conclusion： The aberrant expression of β-catenin and the overexpression of c-myc may be related with the carcinogenesis development of colorectal carcinoma. The aberrant expression of β-catenin may play a role in the development of colorectal carcinoma.