人大肠癌中Ⅳ型胶原酶与基底膜免疫组织化学双重染色研究

An immunohistochemical double labelling study on type Ⅳ collagenase and basement membrane in human colonic carcinoma.

目的观察Ⅳ型胶原酶和基底膜在大肠癌生长中的相互关系及Ⅳ型胶原酶的表达与ｐ２１的表达之间的关系。方法应用免疫组化双标记方法，对８６例人大肠癌组织的Ⅳ型胶原酶表达及基底膜的改变作对照研究。结果大肠癌组织中Ⅳ型胶原酶的阳性率为８３．７２％（７３／８６），与对照组癌周正常肠粘膜的阳性率１０％差异有非常显著意义（Ｐ＜０．０１）。同时，双染色显示Ⅳ型胶原酶阳性的癌组织基底膜表现为不连续，出现缺口、片段缺失或完全消失等形态。此外，肠癌中Ⅳ型胶原酶的分布与ｐ２１的表达呈正相关（ｒ＝０．９７４，ｐ＜０．０１）。结论Ⅳ型胶原酶的分泌对肠癌基底膜破坏起重要作用，可以作为判断大肠癌生长特性的辅助指标。

Objective To investigate the relationship between type Ⅳ collagenase and basement membrane (BM) and the relationship between type Ⅳ collagenase and p21 espression.Methods Immunohistochemical dounle labelling technique was used to study paraffin dmbedded sections of 86 cases of colon carcinoma.Results The positive of type Ⅳ collagenase was 83.72% in carcinoma cases and 10% in normal colorectal mucosa(P<0.01).Intact BM was seen in normal colorectal mucosa while BM was disrupted and fragmentary in carcinoma cases.A close powitve correlation was also round between the expression of type Ⅳ collagenase and p21 in coloniccarcinoma ( r =0.974, P <0.01).Conclusions The results suggest that type Ⅳ collagenase plays an important role in the destruction lf BM and invasion by colonic carcinoma.