蒙古沙鼠非酒精性脂肪性肝病模型的建立与评价

Establishment and Evaluation of Animal Models of Nonalcoholic Fatty Liver Disease in Mongolian Gerbils

目的初步建立并评价蒙古沙鼠非酒精性脂肪性肝病模型。方法选取20只雄性蒙古沙鼠,按体重随机分为模型组及正常对照组,分别予以高脂饲料及正常对照饲料喂养。每周观察两组沙鼠的饮食、体重、皮毛、活动情况、精神状态等一般情况的变化;造模12周后获取血液及肝脏标本,用于血清转氨酶、血脂、血糖等指标的检测以及肝脏病理学的观察,从而评价模型的成模情况。结果模型组的体重明显重于同期正常对照组的体重;造模12周后,模型组血清丙氨酸氨基转移酶、门冬氨酸氨基转移酶、甘油三脂、总胆固醇、血糖等指标均明显高于正常对照组;病理学检查发现,模型组肝脏出现较为明显的脂肪变性,而正常对照组肝脏无异常发现。结论运用高脂饲料喂养蒙古沙鼠12周初步复制了非酒精性脂肪性肝病模型,该模型能较好地模拟出人类非酒精性脂肪性肝病发病的具体病理生理过程以及肝脏病理学变化。

Objective To establish and evaluate the animal model of nonalcoholic fatty liver disese in Mongolian gerbils. Methods Twenty male Mongolian gerbils were randanfly divided into two groups by weight： model group and normal control group, which were treated with high-fat diet and normal control diet respectively. The general status changes in the two group gerbils were observed, including diet, weight, fi.lr, activities and mental status. Twelve weeks after modeling, the blood and liverwere collected to be used as specimen for the detection of serum transaminase, blood fat and blood sugar and the observation of liver histopathology so as to evaluate the modds. Results The weight in model group was obviously higher than in the control group in the same period. Twelve weeks after modeling, the levels of serum alanine aminotransferase, aspartic acid aminotransferase, triglyceride, total bilirubin and blood sugar in the model group were all significantly higher than in the control group. There was obvious liver fatty degeneration in the model group by pathological detection. But there were no obvious changes in the control group. Condusions Mongolian gerbils in the model group fed with high fat diet can develop a potential model of nonalcoholic fatty liver disease, the model could simulate better the pathologic physiology process of the humane nonalcoholic fatty liver disease and the pathological changes in the liver.