异丙酚对大鼠局灶性脑缺血/再灌注损伤的保护作用

Protective Effects of Propofol on Focal Ischemia-reperfusion Injury in Rats

目的探讨异丙酚对大鼠脑缺血/再灌注损伤的保护作用。方法采用改良longa法制备大鼠脑缺血/再灌注模型,随机分为假手术组、对照组、异丙酚组,观察异丙酚对脑缺血/再灌注损伤的保护作用并研究其机制。结果异丙酚明显抑制脑缺血/再灌注后血炎性细胞因子(TNF-α、IL-1β、IL-6、IL-8)升高的程度,改善光镜下脑组织结构的损害,明显升高脑组织超氧化物歧化酶(SOD)的活性,减少丙二醛(MDA)的生成。结论异丙酚抑制脑缺血再灌注时炎性细胞因子的合成和释放,抑制脂质过氧化物MDA的积聚,提高氧化酶SOD的活性,减轻脑组织皮层的损害,减少海马CA1区神经元的死亡,具有脑保护作用。

Objective To explore the protective effects of propofol on cerebral ischemia-reperfusiou injury in rats. Melhods The modified Longa model of foeal cerebral ischemia-reperfusion was used. The healthy mature rats were randomly divided into the sham operation group, the eoutrol gaoup and the propofol ~oup to observe the protective effects of ptopefol on cerebral ischemia-reperfusion injury and to study the mechanism. Results The concentrations of TNF-α、IL-1β.IL-6、IL-8 in propofol group were obviously lower. Propofol could improve the cerebral structure injury under the light microscope, significantly increase the activity of SOD in cerebral tissue, and decrease the generation of MDA. Conclusions Propofol inhibits the synthesis and releases of inflammatory cytokine in the progress of cerebral isehemia-reperfusion, inhibits the accumulation of lipid peroxide MDA, enhances the activity of oxidase SOD, alleviates the cerebral tissue injuries in cortex, and decreases the death of neurons at hippceampal CA1 arat,So propofol has protective effects on cerebra.